{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:29:35Z","timestamp":1790602175066,"version":"4.1.0"},"reference-count":87,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T00:00:00Z","timestamp":1720656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T00:00:00Z","timestamp":1720656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2024,7,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                      is a common cause of healthcare-acquired bloodstream infections and catheter-associated urinary tract infections (CAUTIs) in both adults and children. Treatment of\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      infection is frequently complicated by multi-drug resistance. Based on protein homology,\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      encodes two putative hyaluronidases, EF3023 (HylA) and EF0818 (HylB). In other Gram-positive pathogens, hyaluronidases have been shown to contribute to tissue damage and immune evasion, but the function in\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      has yet to be explored. Here, we show that both\n                      <jats:italic toggle=\"yes\">hylA<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">hylB<\/jats:italic>\n                      contribute to\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      pathogenesis. In a CAUTI model, \u0394\n                      <jats:italic toggle=\"yes\">hylA<\/jats:italic>\n                      exhibited defects in bladder colonization and dissemination to the bloodstream, and \u0394\n                      <jats:italic toggle=\"yes\">hylB<\/jats:italic>\n                      exhibited a defect in kidney colonization. Furthermore, a \u0394\n                      <jats:italic toggle=\"yes\">hylA<\/jats:italic>\n                      \u0394\n                      <jats:italic toggle=\"yes\">hylB<\/jats:italic>\n                      double mutant exhibited a severe colonization defect in a model of bacteremia while the single mutants colonized to a similar level as the wild-type strain, suggesting potential functional redundancy within the bloodstream. We next examined enzymatic activity, and demonstrate that HylB is capable of digesting both hyaluronic acid (HA) and chondroitin sulfate\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      , while HylA exhibits only a very modest activity against heparin. Importantly, HA degradation by HylB provided a modest increase in cell density during the stationary phase and also contributed to dampening of lipopolysaccharide-mediated NF-\u03baB activation. Overall, these data demonstrate that glycosaminoglycan degradation is important for\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      pathogenesis in the urinary tract and during bloodstream infection.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00199-24","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T09:04:21Z","timestamp":1717664661000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Function and contribution of two putative\n                    <i>Enterococcus faecalis<\/i>\n                    glycosaminoglycan degrading enzymes to bacteremia and catheter-associated urinary tract infection"],"prefix":"10.1128","volume":"92","author":[{"given":"Alexandra O.","family":"Johnson","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo","place":["Buffalo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brianna M.","family":"Shipman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Texas at Dallas","place":["Richardson, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin C.","family":"Hunt","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo","place":["Buffalo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian S.","family":"Learman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo","place":["Buffalo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimee L.","family":"Brauer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo","place":["Buffalo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena P.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Texas at Dallas","place":["Richardson, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael","family":"Hageman Blair","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health and Health Professions, State University of New York at Buffalo","place":["Buffalo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7670-5301","authenticated-orcid":true,"given":"Nicole J.","family":"De 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