{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:30:17Z","timestamp":1787016617688,"version":"build-2736575974"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2023,11,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Eimeria intestinalis<\/jats:italic>\n                    is the most pathogenic species of rabbit coccidiosis, causing weight loss, diarrhea, and even acute death. The currently used anticoccidial drugs against\n                    <jats:italic>E. intestinalis<\/jats:italic>\n                    in rabbits are associated with drug resistance and residues. Immunological control might be a potential alternative. We cloned and expressed the\n                    <jats:italic>E. intestinalis<\/jats:italic>\n                    recombinant EF1\u03b1 and EFG (r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EF1\u03b1 and r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EFG, respectively). Rabbits were immunized subcutaneously every 14 days with 100 \u00b5g of r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EF1\u03b1 and r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EFG and followed by 5 \u00d7 10\n                    <jats:sup>4<\/jats:sup>\n                    <jats:italic>E. intestinalis<\/jats:italic>\n                    sporulated oocysts orally challenge. Serum samples were collected every 7 days to measure the levels of specific antibodies and cytokines. On post-challenge day 14, rabbits were sacrificed and the anticoccidial index was evaluated. The rabbits of PBS challenged groups exhibited anorexia, diarrhea, marked intestinal wall thickening, and white nodules that formed patches, while rabbits from the r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EF1\u03b1 or r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EFG challenged group exhibited milder symptoms. The r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EF1\u03b1 group showed a 75.18% oocyst reduction and 89.01%wt gain; the r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EFG group had a 60.58% oocyst reduction and 56.04%wt gain. After vaccination, specific IgG levels increased and stayed high (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). The IL-4 and IL-2 levels of r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EF1\u03b1 immunized groups showed a significant increase after immunization (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). Both r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EF1\u03b1 and r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EFG could induce humoral and cellular immune responses. In contrast, rabbits immunized with r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EF1\u03b1 were better protected from challenge by\n                    <jats:italic>E. intestinalis<\/jats:italic>\n                    than r\n                    <jats:italic>Ei<\/jats:italic>\n                    -EFG.\n                  <\/jats:p>","DOI":"10.1128\/iai.00208-23","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T09:00:21Z","timestamp":1697101221000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Protection against\n                    <i>Eimeria intestinalis<\/i>\n                    infection in rabbits immunized with the recombinant elongation factors EF1\u03b1 and EFG"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4197-5755","authenticated-orcid":false,"given":"Wei","family":"He","sequence":"first","affiliation":[{"name":"Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University , Wenjiang, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University , Wenjiang, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changming","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University , Wenjiang, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University , Wenjiang, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayan","family":"Pu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University , Wenjiang, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University , Wenjiang, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University , Wenjiang, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of 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