{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:40:31Z","timestamp":1785894031885,"version":"3.56.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01AI139267"],"award-info":[{"award-number":["R01AI139267"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R44AI167605"],"award-info":[{"award-number":["R44AI167605"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01AI139267,R44AI167605"],"award-info":[{"award-number":["R01AI139267,R44AI167605"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01AI139267,R44AI167605,R43AI155211"],"award-info":[{"award-number":["R01AI139267,R44AI167605,R43AI155211"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,6,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The efficacy and duration of passive immunity protection depend on maternal antibody levels and transfer efficiency. We investigated whether oral vaccination of\n                      <jats:italic toggle=\"yes\">Peromyscus leucopus<\/jats:italic>\n                      dams with recombinant outer surface protein A (OspA)-expressing\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      could induce maternal transfer of anti-OspA antibodies and protect pups from\n                      <jats:italic toggle=\"yes\">Borrelia burgdorferi<\/jats:italic>\n                      challenge. Dams were vaccinated until breeding pairs were created (i), until parturition (ii), and until pups were 2 weeks old (iii). Pups were challenged with nymphal\n                      <jats:italic toggle=\"yes\">Ixodes scapularis<\/jats:italic>\n                      -transmitted\n                      <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                      at ~4 weeks of age. Anti-OspA IgG was quantified in dams and pups, and anti\n                      <jats:italic toggle=\"yes\">-B<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">burgdorferi<\/jats:italic>\n                      IgG was quantified in pups.\n                      <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                      burden was assessed by\n                      <jats:italic toggle=\"yes\">flaB<\/jats:italic>\n                      quantitative PCR in pups\u2019 tissues ~4 weeks after tick challenge, and viability of\n                      <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                      was assessed by culture of the heart tissue.\n                      <jats:italic toggle=\"yes\">P. leucopus<\/jats:italic>\n                      pups born to dams vaccinated until breeding had low serologic anti-OspA antibody and were not protected from tick-transmitted\n                      <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                      infection. However, when dams\u2019 vaccination extended until parturition and until pups were 2 weeks old, significant anti-OspA antibody transfer and protection from\n                      <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                      infection occurred. This was evidenced by the absence of antibody to\n                      <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                      PepVF, absence of\n                      <jats:italic toggle=\"yes\">B. burgdorferi flaB<\/jats:italic>\n                      DNA in heart and bladder tissues, and absence of\n                      <jats:italic toggle=\"yes\">flaB<\/jats:italic>\n                      in culture from heart tissues from pups euthanized &gt;9 weeks after birth. We show that the transfer of anti-OspA antibodies from vaccinated\n                      <jats:italic toggle=\"yes\">P. leucopus<\/jats:italic>\n                      dams to offspring prevents tick transmission and infection dynamics of\n                      <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                      in the major reservoir host of this spirochete in the USA.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study contributes to our understanding of how interventions based in reservoir-targeted outer surface protein A vaccines designed to block transmission of\n                        <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                        from infected\n                        <jats:italic toggle=\"yes\">Ixodes scapularis<\/jats:italic>\n                        ticks may disrupt the enzootic cycle of this spirochete and reduce incidence of Lyme disease.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00216-25","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T09:20:06Z","timestamp":1747646406000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Maternal transfer of oral vaccine induced anti-OspA antibodies protects\n                    <i>Peromyscus<\/i>\n                    spp. from tick-transmitted\n                    <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"93","author":[{"given":"Jose F.","family":"Azevedo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0011qv509","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology, and Biochemistry, University of Tennessee Health Science 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