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Previously, we showed that a vaccine preparation containing the native hemoglobin receptor HgbA purified from<jats:italic>H. ducreyi<\/jats:italic>class I strain 35000HP (nHgbA<jats:sub>I<\/jats:sub>) and administered with Freund's adjuvant provided complete protection against a homologous challenge. In the current study, we investigated whether nHgbA<jats:sub>I<\/jats:sub>dispensed with monophosphoryl lipid A (MPL), an adjuvant approved for use in humans, offered protection against a challenge with<jats:italic>H. ducreyi<\/jats:italic>strain 35000HP expressing either class I or class II HgbA (35000HP<jats:italic>hgbA<jats:sub>I<\/jats:sub><\/jats:italic>and 35000HP<jats:italic>hgbA<jats:sub>II<\/jats:sub><\/jats:italic>, respectively). Pigs immunized with the nHgbA<jats:sub>I<\/jats:sub>\/MPL vaccine were protected against a challenge from homologous<jats:italic>H. ducreyi<\/jats:italic>strain 35000HP<jats:italic>hgbA<jats:sub>I<\/jats:sub><\/jats:italic>but not heterologous strain 35000HP<jats:italic>hgbA<jats:sub>II<\/jats:sub><\/jats:italic>, as evidenced by the isolation of only strain 35000HP<jats:italic>hgbA<jats:sub>II<\/jats:sub><\/jats:italic>from nHgbA<jats:sub>I<\/jats:sub>-immunized pigs. Furthermore, histological analysis of the lesions showed striking differences between mock-immunized and nHgbA<jats:sub>I<\/jats:sub>-immunized animals challenged with strains 35000HP<jats:italic>hgbA<jats:sub>I<\/jats:sub><\/jats:italic>but not those challenged with strain 35000HP<jats:italic>hgbA<jats:sub>II<\/jats:sub><\/jats:italic>. Mock-immunized pigs were not protected from a challenge by either strain. The enzyme-linked immunosorbent assay (ELISA) activity of the nHgbA<jats:sub>I<\/jats:sub>\/MPL antiserum was lower than the activity of antiserum from animals immunized with the nHgbA<jats:sub>I<\/jats:sub>\/Freund's vaccine; however, anti-nHgbA<jats:sub>I<\/jats:sub>from both studies bound whole cells of 35000HP<jats:italic>hgbA<jats:sub>I<\/jats:sub><\/jats:italic>better than 35000HP<jats:italic>hgbA<jats:sub>II<\/jats:sub><\/jats:italic>and partially blocked hemoglobin binding to nHgbA<jats:sub>I<\/jats:sub>. In conclusion, despite eliciting lower antibody ELISA activity than the nHgbA<jats:sub>I<\/jats:sub>\/Freund's, the nHgbA<jats:sub>I<\/jats:sub>\/MPL vaccine provided protection against a challenge with homologous but not heterologous<jats:italic>H. ducreyi<\/jats:italic>, suggesting that a bivalent HgbA vaccine may be needed.<\/jats:p>","DOI":"10.1128\/iai.00217-10","type":"journal-article","created":{"date-parts":[[2010,6,29]],"date-time":"2010-06-29T04:19:20Z","timestamp":1277785160000},"page":"3763-3772","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Immunization with the<i>Haemophilus ducreyi<\/i>Hemoglobin Receptor HgbA with Adjuvant Monophosphoryl Lipid A Protects Swine from a Homologous but Not a Heterologous Challenge"],"prefix":"10.1128","volume":"78","author":[{"given":"William G.","family":"Fusco","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Departments of 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