{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:13:40Z","timestamp":1792707220009,"version":"4.5.0"},"reference-count":72,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R00AI106963"],"award-info":[{"award-number":["R00AI106963"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007588","name":"Washington State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007588","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n            is the causative agent of Q fever, a zoonotic disease that threatens both human and animal health. Due to the paucity of experimental animal models, little is known about how host factors interface with bacterial components and affect pathogenesis. Here, we used\n            <jats:named-content content-type=\"genus-species\">Drosophila melanogaster<\/jats:named-content>\n            , in conjunction with the biosafety level 2 (BSL2) Nine Mile phase II (NMII) clone 4 strain of\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            , as a model to investigate host and bacterial components implicated in infection. We demonstrate that adult\n            <jats:named-content content-type=\"genus-species\">Drosophila<\/jats:named-content>\n            flies are susceptible to\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            NMII infection and that this bacterial strain, which activates the immune deficiency (IMD) pathway, is able to replicate and cause mortality in the animals. We show that in the absence of Eiger, the only known tumor necrosis factor (TNF) superfamily homolog in\n            <jats:named-content content-type=\"genus-species\">Drosophila<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            -infected flies exhibit reduced mortality from infection. We also demonstrate that the\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            type 4 secretion system (T4SS) is critical for the formation of the\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            -containing vacuole and establishment of infection in\n            <jats:named-content content-type=\"genus-species\">Drosophila<\/jats:named-content>\n            . Altogether, our data reveal that the\n            <jats:named-content content-type=\"genus-species\">Drosophila<\/jats:named-content>\n            TNF homolog Eiger and the\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            T4SS are implicated in the pathogenesis of\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            in flies. The\n            <jats:named-content content-type=\"genus-species\">Drosophila<\/jats:named-content>\n            \/NMII model mimics relevant aspects of the infection in mammals, such as a critical role of host TNF and the bacterial T4SS in pathogenesis. Our work also demonstrates the usefulness of this BSL2 model to investigate both host and\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            components implicated in infection.\n          <\/jats:p>","DOI":"10.1128\/iai.00218-17","type":"journal-article","created":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T00:25:27Z","timestamp":1493079927000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Host and Bacterial Factors Control Susceptibility of Drosophila melanogaster to Coxiella burnetii Infection"],"prefix":"10.1128","volume":"85","author":[{"given":"Reginaldo G.","family":"Bastos","sequence":"first","affiliation":[{"name":"School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary 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