{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:18:00Z","timestamp":1762917480271},"reference-count":40,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella melitensis<\/jats:named-content>\n            causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. Attenuated\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. melitensis<\/jats:named-content>\n            strain M5-90, derived from virulent strain M28, is widely used as a live vaccine in ruminants in China. Genetic differences between the strains may cast light on the mechanism of attenuation. We recently reported the complete genomic sequences of M28 and M5-90. Genome organization is highly conserved between these isolates, and also with virulent strains 16 M and ATCC 23457. Analysis revealed 23 open reading frames (ORFs) with consistent differences between M5-90 and the virulent strains. Notably, the\n            <jats:italic>tuf2<\/jats:italic>\n            gene encoding translation elongation factor EF-Tu from M5-90 contained 50 single nucleotide polymorphisms (SNPs) and 9 gaps (indels) compared to\n            <jats:italic>tuf2<\/jats:italic>\n            of M28 or of the other virulent strains. There were no changes in\n            <jats:italic>tuf1<\/jats:italic>\n            . To evaluate the potential role of EF-Tu in pathogenesis,\n            <jats:italic>tuf1<\/jats:italic>\n            and\n            <jats:italic>tuf2<\/jats:italic>\n            mutants of M28 and an M5-90 strain harboring wild-type\n            <jats:italic>tuf2<\/jats:italic>\n            were constructed, and their virulence\/attenuation was evaluated\n            <jats:italic>in vivo<\/jats:italic>\n            . We report that the\n            <jats:italic>tuf2<\/jats:italic>\n            gene plays an important role in the attenuation of M5-90 virulence.\n          <\/jats:p>","DOI":"10.1128\/iai.00224-13","type":"journal-article","created":{"date-parts":[[2013,5,29]],"date-time":"2013-05-29T02:13:14Z","timestamp":1369793594000},"page":"2812-2818","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Comparison of Genomes of Brucella melitensis M28 and the B. melitensis M5-90 Derivative Vaccine Strain Highlights the Translation Elongation Factor Tu Gene\n            <i>tuf2<\/i>\n            as an Attenuation-Related Gene"],"prefix":"10.1128","volume":"81","author":[{"given":"Fangkun","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Veterinary Biotechnology and Zoonosis Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China"},{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zujian","family":"Qiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Biotechnology and Zoonosis Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sen","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Biotechnology and Zoonosis Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxing","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Biotechnology and Zoonosis Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huajun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shanghai-Ministry Key Laboratory of Disease and Health Genomics, Chinese National Human Genome Center, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidang","family":"Liu","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigao","family":"Bu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Biotechnology and Zoonosis Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.06.021"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00252-3"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/biol.1998.0158"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970201"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01767-08"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.11.023"},{"key":"e_1_3_3_9_2","first-page":"65","article-title":"Study on the Brucella melitensis strain M5-90 vaccine","volume":"6","author":"Research Group of Brucellosis (Harbin Veterinary Research Institute)","year":"1991","unstructured":"Research Group of Brucellosis (Harbin Veterinary Research Institute). 1991. 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