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The\n            <jats:italic>Y. pestis<\/jats:italic>\n            adhesin Ail mediates host cell binding and is critical for Yop delivery. To identify the Ail receptor(s), Ail was purified following overexpression in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . Ail bound specifically to fibronectin, an extracellular matrix protein with the potential to act as a bridge between Ail and host cells. Ail expressed by\n            <jats:italic>E. coli<\/jats:italic>\n            also mediated binding to purified fibronectin, and Ail-mediated\n            <jats:italic>E. coli<\/jats:italic>\n            adhesion to host cells was dependent on fibronectin. Ail expressed by\n            <jats:italic>Y. pestis<\/jats:italic>\n            bound purified fibronectin, as did the\n            <jats:italic>Y. pestis<\/jats:italic>\n            adhesin plasminogen activator (Pla). However, a KIM5 \u0394\n            <jats:italic>ail<\/jats:italic>\n            mutant had decreased binding to host cells, while a KIM5 \u0394\n            <jats:italic>pla<\/jats:italic>\n            mutant had no significant defect in adhesion. Furthermore, treatment with antifibronectin antibodies decreased Ail-mediated adhesion by KIM5 and the KIM5 \u0394\n            <jats:italic>pla<\/jats:italic>\n            mutant, indicating that the Ail-fibronectin interaction was important for cell binding. Finally, antifibronectin antibodies inhibited the KIM5-mediated cytotoxicity of host cells in an Ail-dependent fashion. These data indicate that Ail is a key adhesin that mediates binding to host cells through interaction with fibronectin on the surface of host cells, and this interaction is important for Yop delivery by\n            <jats:italic>Y. pestis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00238-10","type":"journal-article","created":{"date-parts":[[2010,5,25]],"date-time":"2010-05-25T02:23:54Z","timestamp":1274754234000},"page":"3358-3368","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Ail Binding to Fibronectin Facilitates\n            <i>Yersinia pestis<\/i>\n            Binding to Host Cells and Yop Delivery"],"prefix":"10.1128","volume":"78","author":[{"given":"Tiffany M.","family":"Tsang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suleyman","family":"Felek","sequence":"additional","affiliation":[{"name":"Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.","family":"Krukonis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan School of Medicine"},{"name":"Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408026101"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89291-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01125-07"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02021.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.8.3561"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00790.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.17.5298-5307.1991"},{"key":"e_1_3_2_9_2","first-page":"227","year":"2001","unstructured":"Boyd, A. 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