{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T15:51:18Z","timestamp":1775231478570,"version":"3.50.1"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            , the plague pathogen, is a facultative intracellular bacterium. Cellular immunity plays important roles in defense against infections. The identification of T-cell targets is critical for the development of effective vaccines against intracellular bacteria; however, the function of cellular immunity in protection from plague was not clearly understood. In this study, 261 genes from\n            <jats:italic>Y. pestis<\/jats:italic>\n            were selected on the basis of bioinformatics analysis and previous research results for expression in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            BL21(DE3). After purification, 101 proteins were qualified for examination of their abilities to induce the production of gamma interferon in mice immunized with live vaccine EV76 by enzyme-linked immunospot assay. Thirty-four proteins were found to stimulate strong T-cell responses. The protective efficiencies for 24 of them were preliminarily evaluated using a mouse plague model. In addition to LcrV, nine proteins (YPO0606, YPO1914, YPO0612, YPO3119, YPO3047, YPO1377, YPCD1.05c, YPO0420, and YPO3720) may provide partial protection against challenge with a low dose (20 times the 50% lethal dose [20\u00d7 LD\n            <jats:sub>50<\/jats:sub>\n            ]) of\n            <jats:italic>Y. pestis<\/jats:italic>\n            , but only YPO0606 could partially protect mice from infection with\n            <jats:italic>Y. pestis<\/jats:italic>\n            at a higher challenge dosage (200\u00d7 LD\n            <jats:sub>50<\/jats:sub>\n            ). These proteins would be the potential components for\n            <jats:italic>Y. pestis<\/jats:italic>\n            vaccine development.\n          <\/jats:p>","DOI":"10.1128\/iai.00242-09","type":"journal-article","created":{"date-parts":[[2009,8,4]],"date-time":"2009-08-04T16:07:37Z","timestamp":1249402057000},"page":"4356-4361","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["High-Throughput Identification of New Protective Antigens from a\n            <i>Yersinia pestis<\/i>\n            Live Vaccine by Enzyme-Linked Immunospot Assay"],"prefix":"10.1128","volume":"77","author":[{"given":"Bei","family":"Li","sequence":"first","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"},{"name":"Yunyang Medical College, Shiyan, Hubei Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JingYu","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Ni","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuehua","family":"Ke","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziwen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaobiao","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruifu","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.11.020"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.11.4580-4585.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.3.1533-1537.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.01.076"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1922-1928.1999"},{"key":"e_1_3_2_7_2","first-page":"804","volume":"32","year":"2003","unstructured":"Bossi, P., and F. 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