{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:22:52Z","timestamp":1762917772283,"version":"3.41.2"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI031338"],"award-info":[{"award-number":["AI031338"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000090","name":"DOD | United States Army | MEDCOM | Congressionally Directed Medical Research Programs","doi-asserted-by":"publisher","award":["W81XWH-12-1-0403"],"award-info":[{"award-number":["W81XWH-12-1-0403"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n A potent vaccine against tuberculosis, one of the world's deadliest diseases, is needed to enhance the immunity of people worldwide, most of whom have been vaccinated with the partially effective\n Mycobacterium bovis<\/jats:named-content>\n BCG vaccine. Here we investigate novel live attenuated recombinant\n Listeria monocytogenes<\/jats:named-content>\n (rLm) vaccines expressing the\n Mycobacterium tuberculosis<\/jats:named-content>\n 30-kDa major secretory protein (r30\/antigen 85B [Ag85B]) (rLm30) as heterologous booster vaccines in animals primed with BCG. Using three attenuated\n L. monocytogenes<\/jats:named-content>\n vectors,\n L. monocytogenes<\/jats:named-content>\n \u0394\n actA<\/jats:italic>\n (LmI),\n L. monocytogenes<\/jats:named-content>\n \u0394\n actA<\/jats:italic>\n \u0394\n inlB<\/jats:italic>\n (LmII), and\n L. monocytogenes<\/jats:named-content>\n \u0394\n actA<\/jats:italic>\n \u0394\n inlB prfA<\/jats:italic>\n * (LmIII), we constructed five rLm30 vaccine candidates expressing r30 linked in frame to the\n L. monocytogenes<\/jats:named-content>\n listeriolysin O signal sequence and driven by the\n hly<\/jats:italic>\n promoter (h30) or linked in frame to the ActA N-terminal 100 amino acids and driven by the\n actA<\/jats:italic>\n promoter (a30). All five rLm30 vaccines secreted r30 in broth and macrophages; while rLm30 expressing r30 via a constitutively active\n prfA<\/jats:italic>\n * regulon (rLmIII\/a30) expressed the largest amount of r30 in broth culture, all five rLm30 vaccines expressed equivalent amounts of r30 in infected macrophages. In comparative studies, boosting of BCG-immunized mice with rLmIII\/a30 induced the strongest antigen-specific T-cell responses, including splenic and lung polyfunctional CD4\n +<\/jats:sup>\n T cells expressing the three cytokines interferon gamma (IFN-\u03b3), tumor necrosis factor alpha (TNF-\u03b1), and interleukin-2 (IL-2) (\n P<\/jats:italic>\n < 0.001) and splenic and lung CD8\n +<\/jats:sup>\n T cells expressing IFN-\u03b3 (\n P<\/jats:italic>\n < 0.0001). In mice and guinea pigs, the rLmIII\/a30 and rLmI\/h30 vaccines were generally more potent booster vaccines than r30 with an adjuvant and a recombinant adenovirus vaccine expressing r30. In a setting in which BCG alone was highly immunoprotective, boosting of mice with rLmIII\/a30, the most potent of the vaccines, significantly enhanced protection against aerosolized\n M. tuberculosis<\/jats:named-content>\n (\n P<\/jats:italic>\n < 0.01).\n <\/jats:p>","DOI":"10.1128\/iai.00245-17","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T00:40:37Z","timestamp":1497919237000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Listeria-Vectored Vaccine Expressing the Mycobacterium tuberculosis 30-Kilodalton Major Secretory Protein via the Constitutively Active\n prfA<\/i>\n * Regulon Boosts Mycobacterium bovis BCG Efficacy against Tuberculosis"],"prefix":"10.1128","volume":"85","author":[{"given":"Qingmei","family":"Jia","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of California\u2014Los Angeles, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara Jane","family":"Dillon","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of California\u2014Los Angeles, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sa\u0161a","family":"Masle\u0161a-Gali\u0107","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of California\u2014Los Angeles, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus A.","family":"Horwitz","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of California\u2014Los Angeles, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Global tuberculosis report 2016","author":"WHO","year":"2016","unstructured":"WHO. 2016. 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