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Among vaccine targets, the lipopolysaccharide (LPS) O antigen of\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            is the most immunodominant protective candidate. There are 20 different O antigens composed of different repeat sugar structures conferring serogroup specificity, and 10 are found most frequently in infection. Thus, one approach to combat infection by\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            could be to generate immunity with a vaccine cocktail that includes all these serogroups. Serogroup O9 is 1 of the 10 serogroups commonly found in infection, but it has never been developed into a vaccine, due in part to the acid-labile nature of the O9 polysaccharide. Our laboratory has previously shown that intranasal administration of an attenuated\n            <jats:italic>Salmonella<\/jats:italic>\n            strain expressing the\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            serogroup O11 LPS O antigen was effective in clearing bacteria and preventing mortality in mice following intranasal challenge with serogroup O11\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            . Consequently, we set out to develop a\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            serogroup O9 vaccine using a similar approach. Here, we show that\n            <jats:italic>Salmonella<\/jats:italic>\n            expressing serogroup O9 triggered an antibody-mediated immune response following intranasal administration to mice and that it conferred protection from\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            serogroup O9 in a murine model of acute pneumonia.\n          <\/jats:p>","DOI":"10.1128\/iai.00247-23","type":"journal-article","created":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T14:05:14Z","timestamp":1700661914000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Efficacy of a\n            <i>Pseudomonas aeruginosa<\/i>\n            serogroup O9 vaccine"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9616-5947","authenticated-orcid":false,"given":"Dina A.","family":"Moustafa","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division 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Laney Graduate School, Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Schulman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Scarff","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Michael R.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John 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