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Previous investigations focused on the pro-inflammatory effects of leukocytes and the pivotal role of epithelial cell metabolic status in finely modulating the inflammatory response have been neglected. Herein, we examined how glycolysis in airway epithelial cells is affected by\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    infection in an\n                    <jats:italic>in vitro<\/jats:italic>\n                    model. Additionally, we investigated the contribution of ATP to pulmonary inflammation. Metabolic analysis revealed a marked metabolic shift in bronchial epithelial cells during\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    infection, characterized by increased glucose uptake, enhanced aerobic glycolysis, and augmented ATP synthesis. Notably, these metabolic alterations are orchestrated by adaptor proteins, MyD88 and TRAM. The resulting synthesized ATP is released into the extracellular milieu via vesicular exocytosis and pannexin protein channels, leading to a substantial increase in extracellular ATP levels. The conditioned medium supernatant from\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    -infected epithelial cells enhances the secretion of both interleukin (IL)-1\u03b2 and IL-18 by peripheral blood mononuclear cells, partially mediated by the P2X7 purine receptor (P2X7R).\n                    <jats:italic>In vivo<\/jats:italic>\n                    experiments confirm that addition of a conditioned medium exacerbates pulmonary inflammation, which can be attenuated by pre-treatment with a P2X7R inhibitor. Collectively, these findings highlight the significance of airway epithelial aerobic glycolysis in enhancing the pulmonary inflammatory response and aiding pathogen clearance.\n                  <\/jats:p>","DOI":"10.1128\/iai.00248-23","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T08:30:47Z","timestamp":1704961847000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Aerobic glycolysis of bronchial epithelial cells rewires\n                    <i>Mycoplasma pneumoniae<\/i>\n                    pneumonia and promotes bacterial elimination"],"prefix":"10.1128","volume":"92","author":[{"given":"Jun","family":"He","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical College, University of South China, Hengyang, China"},{"name":"Institute of Pathogenic Biology, Hengyang Medical College, Hunan 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