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Our understanding of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. pneumophila<\/jats:named-content>\n            pathogenicity is obtained mostly from study of derivatives of several clinical isolates, which employ almost identical virulent determinants to exploit host functions. To determine whether environmental\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. pneumophila<\/jats:named-content>\n            isolates interact similarly with the model host systems, we analyzed intracellular replication of several recently isolated such strains and found that these strains cannot productively grow in bone marrow-derived macrophages of A\/J mice, which are permissive for all examined laboratory strains. By focusing on one strain called LPE509, we found that its deficiency in intracellular replication in primary A\/J macrophages is not caused by the lack of important pathogenic determinants because this strain replicates proficiently in two protozoan hosts and the human macrophage U937 cell. We also found that in the early phase of infection, the trafficking of this strain in A\/J macrophages is similar to that of JR32, a derivative of strain Philadelphia 1. Furthermore, infection of these cells by LPE509 caused extensive cell death in a process that requires the Dot\/Icm type IV secretion system. Finally, we showed that the cell death is caused neither by the activation of the NAIP5\/NLRC4 inflammasome nor by the recently described caspase 11-dependent pathway. Our results revealed that some environmental\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. pneumophila<\/jats:named-content>\n            strains are unable to overcome the defense conferred by primary macrophages from mice known to be permissive for laboratory\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. pneumophila<\/jats:named-content>\n            strains. These results also suggest the existence of a host immune surveillance mechanism differing from those currently known in responding to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. pneumophila<\/jats:named-content>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.00252-13","type":"journal-article","created":{"date-parts":[[2013,6,11]],"date-time":"2013-06-11T03:17:08Z","timestamp":1370920628000},"page":"3077-3088","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Induction of Rapid Cell Death by an Environmental Isolate of Legionella pneumophila in Mouse Macrophages"],"prefix":"10.1128","volume":"81","author":[{"given":"Lili","family":"Tao","sequence":"first","affiliation":[{"name":"Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, 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