{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:44:34Z","timestamp":1791186274621,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2008,8,1]],"date-time":"2008-08-01T00:00:00Z","timestamp":1217548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Mycoplasma genitalium<\/jats:italic>is a sexually transmitted bacterial pathogen that causes nongonococcal chlamydia-negative urethritis, mucopurulent cervicitis, endometritis, pelvic inflammatory disease, and tubal factor infertility in humans. However, pathogenic agents that induce inflammatory responses have not been identified in<jats:italic>M. genitalium<\/jats:italic>. In this study, we examined the involvement of Toll-like receptors (TLRs) in activation of the immune response by a lipoprotein from<jats:italic>M. genitalium<\/jats:italic>and their active component responsible for NF-\u03baB activation. The Triton X-114 detergent phase of<jats:italic>M. genitalium<\/jats:italic>was found to induce NF-\u03baB through TLR2. The active component of the Triton X-114 detergent phase was a lipoprotein precursor, MG149. The activation of NF-\u03baB by MG149 was inhibited by a dominant negative (DN) construct of TLR1 but not by a DN construct of TLR6. These results indicate that the activation of NF-\u03baB by MG149 is dependent on TLR1 and TLR2. A synthetic lipopeptide derived from MG149 containing three acyl chains also induced NF-\u03baB through TLR1 and TLR2. Thus, the results show that MG149, a triacylated lipoprotein from<jats:italic>M. genitalium<\/jats:italic>, activates NF-\u03baB through TLR1 and TLR2.<\/jats:p>","DOI":"10.1128\/iai.00257-08","type":"journal-article","created":{"date-parts":[[2008,5,13]],"date-time":"2008-05-13T00:45:27Z","timestamp":1210639527000},"page":"3672-3678","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["A Triacylated Lipoprotein from<i>Mycoplasma genitalium<\/i>Activates NF-\u03baB through Toll-Like Receptor 1 (TLR1) and TLR2"],"prefix":"10.1128","volume":"76","author":[{"given":"Takashi","family":"Shimizu","sequence":"first","affiliation":[{"name":"The Division of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Kida","sequence":"additional","affiliation":[{"name":"The Division of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Kuwano","sequence":"additional","affiliation":[{"name":"The Division of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1391"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5428.736"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/095646240001100504"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5428.732"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(99)01641-8"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/16.9.1866"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07848-0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65268-8"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.9.3916-3921.1994"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.6.2951-2956.1999"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5235.397"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/35074106"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/35047123"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.22.4420"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.9.4531"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"3749","DOI":"10.4049\/jimmunol.162.7.3749","volume":"162","year":"1999","unstructured":"Hoshino, K., O. 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