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To deliver the candidate antigen on the surface, we fused the\n                    <jats:italic>mpt64<\/jats:italic>\n                    gene to the sequence encoding the PE domain of the PE_PGRS33 protein of\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    (to create strain\n                    <jats:sub>H<\/jats:sub>\n                    <jats:italic>PE<\/jats:italic>\n                    -\u0394MPT64-BCG), which we have previously shown to transport proteins to the bacterial surface. In a series of protection experiments in the mouse model of tuberculosis, we showed that (i) immunization of mice with\n                    <jats:sub>H<\/jats:sub>\n                    <jats:italic>PE<\/jats:italic>\n                    -\u0394MPT64-BCG provides levels of protection significantly higher than those afforded by the parental BCG strain, as assessed by bacterial colonization in lungs and spleens and by lung involvement (at both 28 and 70 days postchallenge), (ii) rBCG strains expressing MPT64 provide better protection than the parental BCG strain only when this antigen is surface expressed, and (iii) the\n                    <jats:sub>H<\/jats:sub>\n                    <jats:italic>PE<\/jats:italic>\n                    -\u0394MPT64-BCG-induced MPT64-specific T cell repertoire when characterized by \u03b2 chain variable region-\u03b2 chain joining region (BV-BJ) spectratyping indicates that protection is correlated with the ability to recruit gamma interferon (IFN-\u03b3)-secreting T cells carrying the BV8.3-BJ1.5 (172 bp) shared rearrangement. These results demonstrate that\n                    <jats:sub>H<\/jats:sub>\n                    <jats:italic>PE<\/jats:italic>\n                    -\u0394MPT64-BCG is one of the most effective new vaccines tested so far in the mouse model of tuberculosis and underscore the impact of antigen cellular localization on the induction of the specific immune response induced by rBCG.\n                  <\/jats:p>","DOI":"10.1128\/iai.00267-10","type":"journal-article","created":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T23:20:04Z","timestamp":1286234404000},"page":"5202-5213","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Surface Expression of MPT64 as a Fusion with the PE Domain of PE_PGRS33 Enhances\n                    <i>Mycobacterium bovis<\/i>\n                    BCG Protective Activity against\n                    <i>Mycobacterium tuberculosis<\/i>\n                    in Mice"],"prefix":"10.1128","volume":"78","author":[{"given":"Michela","family":"Sali","sequence":"first","affiliation":[{"name":"Institute of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Di Sante","sequence":"additional","affiliation":[{"name":"Institute of General Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Cascioferro","sequence":"additional","affiliation":[{"name":"Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via A. 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