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Current acellular pertussis vaccines, though having favorable reactogenicity and efficacy profiles, involve complex and costly production processes. In addition, acellular vaccines have functional challenges such as short-lasting duration of immunity and limited antigen coverage. Filamentous hemagglutinin (FHA) is an adhesin of\n              <jats:italic>B. pertussis<\/jats:italic>\n              that is included in all multivalent pertussis vaccine formulations. Antibodies to FHA have been shown to prevent bacterial attachment to respiratory epithelial cells, and T cell responses to FHA facilitate cell-mediated immunity. In this study, FHA\u2019s mature C-terminal domain (MCD) was evaluated as a novel vaccine antigen. MCD was conjugated to virus-like particles via SpyTag-SpyCatcher technology. Prime-boost vaccine studies were performed in mice to characterize immunogenicity and protection against the intranasal\n              <jats:italic>B. pertussis<\/jats:italic>\n              challenge. MCD-SpyVLP was more immunogenic than SpyTag-MCD antigen alone, and in Tohama I strain challenge studies, improved protection against challenge was observed in the lungs at day 3 and in the trachea and nasal wash at day 7 post-challenge. Furthermore, a\n              <jats:italic>B. pertussis<\/jats:italic>\n              strain encoding genetically inactivated pertussis toxin was used to evaluate MCD-SpyVLP vaccine immunity. Mice vaccinated with MCD-SpyVLP had significantly lower respiratory bacterial burden at both days 3 and 7 post-challenge compared to mock-vaccinated animals. Overall, these data support the use of SpyTag-SpyCatcher VLPs as a platform for use in vaccine development against\n              <jats:italic>B. pertussis<\/jats:italic>\n              and other pathogens.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00270-24","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T13:00:28Z","timestamp":1721307628000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Virus-like particles displaying the mature C-terminal domain of filamentous hemagglutinin are immunogenic and protective against\n            <i>Bordetella pertussis<\/i>\n            respiratory infection in mice"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5782-5658","authenticated-orcid":false,"given":"Gage M.","family":"Pyles","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and 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