{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T16:35:32Z","timestamp":1792427732154,"version":"4.5.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In humans with typhoid fever or in mouse strains susceptible to<jats:italic>Salmonella enterica<\/jats:italic>serovar Typhimurium (<jats:italic>S<\/jats:italic>. Typhimurium) infection, bacteria gain access to extraintestinal tissues, causing severe systemic disease. Here we show that in the gut-draining mesenteric lymph nodes (MLN), the majority of<jats:italic>S.<\/jats:italic>Typhimurium-carrying cells show dendritic-cell (DC) morphology and express the DC marker CD11c, indicating that<jats:italic>S<\/jats:italic>. Typhimurium bacteria are transported to the MLN by migratory DCs. In vivo FLT-3L-induced expansion of DCs, as well as stimulation of DC migration by Toll-like receptor agonists, results in increased numbers of<jats:italic>S<\/jats:italic>. Typhimurium bacteria reaching the MLN. Conversely, genetically impaired DC migration in chemokine receptor CCR7-deficient mice reduces the number of<jats:italic>S<\/jats:italic>. Typhimurium bacteria reaching the MLN. This indicates that transport of<jats:italic>S<\/jats:italic>. Typhimurium from the intestine into the MLN is limited by the number of migratory DCs carrying<jats:italic>S<\/jats:italic>. Typhimurium bacteria. In contrast, modulation of DC migration does not affect the number of<jats:italic>S<\/jats:italic>. Typhimurium bacteria reaching systemic tissues, indicating that DC-bound transport of<jats:italic>S<\/jats:italic>. Typhimurium does not substantially contribute to systemic<jats:italic>S<\/jats:italic>. Typhimurium infection. Surgical removal of the MLN results in increased numbers of<jats:italic>S<\/jats:italic>. Typhimurium bacteria reaching systemic sites early after infection, thereby rendering otherwise resistant mice susceptible to fatal systemic disease development. This suggests that the MLN provide a vital barrier shielding systemic compartments from DC-mediated dissemination of<jats:italic>S<\/jats:italic>. Typhimurium. Thus, confinement of<jats:italic>S<\/jats:italic>. Typhimurium in gut-associated lymphoid tissue and MLN delays massive extraintestinal dissemination and at the same time allows for the establishment of protective adaptive immune responses.<\/jats:p>","DOI":"10.1128\/iai.00272-09","type":"journal-article","created":{"date-parts":[[2009,6,9]],"date-time":"2009-06-09T01:28:56Z","timestamp":1244510936000},"page":"3170-3180","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":121,"title":["Mesenteric Lymph Nodes Confine Dendritic Cell-Mediated Dissemination of<i>Salmonella enterica<\/i>Serovar Typhimurium and Limit Systemic Disease in Mice"],"prefix":"10.1128","volume":"77","author":[{"given":"Sabrina","family":"Voedisch","sequence":"first","affiliation":[{"name":"Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Koenecke","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"David","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Herbrand","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhold","family":"Fo\u0308rster","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Rhen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, 17177 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Pabst","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20060905"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.4049\/jimmunol.144.8.3143","volume":"144","year":"1990","unstructured":"Benjamin, W. H., Jr., P. Hall, S. J. Roberts, and D. E. Briles. 1990. The primary effect of the Ity locus is on the rate of growth of Salmonella typhimurium that are relatively protected from killing. J. Immunol.144:3143-3151.","journal-title":"J. Immunol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00150.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02821.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.139.5.1189"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80059-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.16.6383"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030196"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.08.002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01392-06"},{"key":"e_1_3_2_12_2","first-page":"437","volume":"205","year":"2008","unstructured":"Hapfelmeier, S., A. J. Muller, B. Stecher, P. Kaiser, M. Barthel, K. Endt, M. Eberhard, R. Robbiani, C. A. Jacobi, M. Heikenwalder, C. Kirschning, S. Jung, T. Stallmach, M. Kremer, and W. D. Hardt. 2008. Microbe sampling by mucosal dendritic cells is a discrete, MyD88-independent step in \u0394invG S. Typhimurium colitis. J. Exp. Med.205:437-450.","journal-title":"Typhimurium colitis. J. Exp. Med."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7618105"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v28.i1.10"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/291238a0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400969101"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051100"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.15"},{"key":"e_1_3_2_19_2","first-page":"3239","volume":"60","year":"2000","unstructured":"Mach, N., S. Gillessen, S. B. Wilson, C. Sheehan, M. Mihm, and G. Dranoff. 2000. Differences in dendritic cells stimulated in vivo by tumors engineered to secrete granulocyte-macrophage colony-stimulating factor or Flt3-ligand. Cancer Res.60:3239-3246.","journal-title":"Cancer Res."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1091334"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.10.011"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.2174\/1566524023362492"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00289-3"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3344-3348.2000"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102901"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.15.7800"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2004.06.014"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00784-07"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/86373"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.4.569"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.9.5789"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.6.2593"},{"key":"e_1_3_2_33_2","first-page":"109","volume":"50","year":"1967","unstructured":"Takeuchi, A. 1967. Electron microscope studies of experimental Salmonella infection. I. Penetration into the intestinal epithelium by Salmonella typhimurium. Am. J. Pathol50:109-136.","journal-title":"I. Penetration into the intestinal epithelium by Salmonella typhimurium. Am. J. Pathol"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00679.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1362"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01155.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/44593"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052016"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604054103"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636426"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.9.5205"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00272-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00272-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T16:16:26Z","timestamp":1685031386000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00272-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["10.1128\/IAI.00272-09"],"URL":"https:\/\/doi.org\/10.1128\/iai.00272-09","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8]]},"assertion":[{"value":"2009-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-05-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}