{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:52:22Z","timestamp":1790815942699,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study we tested the in vitro and in vivo anti-\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    activity of an antilaminarin (anti-\u03b2-glucan) monoclonal antibody (MAb 2G8) (immunoglobulin G2b) which was previously shown to inhibit the growth of \u03b2-glucan-exposing\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    cells. Here we show that MAb 2G8 binds to the cell wall of\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    and inhibits its growth to an extent comparable to that observed for\n                    <jats:italic>C. albicans<\/jats:italic>\n                    . Binding and growth inhibition were detected almost equally for encapsulated and acapsular\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    strains. In addition, at subinhibitory concentrations, MAb 2G8 reduced the capsule thickness without affecting protease or phospholipase production. Acapsular fungal cells, but not encapsulated fungal cells, were opsonized by the antibody and more efficiently phagocytosed and killed by human monocytes and by murine peritoneal macrophages. A single administration of MAb 2G8 resulted in a reduction in the fungal burden in the brains and livers of mice systemically infected with a highly virulent, encapsulated\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    strain. This protective effect was also detected in neutropenic mice. Overall, these findings demonstrate that cell wall \u03b2-glucan of encapsulated\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    is accessible to antibodies which can exert remarkable anticryptococcal activities in vitro and in vivo.\n                  <\/jats:p>","DOI":"10.1128\/iai.00278-07","type":"journal-article","created":{"date-parts":[[2007,7,2]],"date-time":"2007-07-02T20:34:01Z","timestamp":1183408441000},"page":"5085-5094","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":151,"title":["An Anti-\u03b2-Glucan Monoclonal Antibody Inhibits Growth and Capsule Formation of\n                    <i>Cryptococcus neoformans<\/i>\n                    In Vitro and Exerts Therapeutic, Anticryptococcal Activity In Vivo"],"prefix":"10.1128","volume":"75","author":[{"given":"Anna","family":"Rachini","sequence":"first","affiliation":[{"name":"Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06126 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Pietrella","sequence":"additional","affiliation":[{"name":"Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06126 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Lupo","sequence":"additional","affiliation":[{"name":"Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06126 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Torosantucci","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita\u0300, 00186 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Chiani","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita\u0300, 00186 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Bromuro","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita\u0300, 00186 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Proietti","sequence":"additional","affiliation":[{"name":"Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06126 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Bistoni","sequence":"additional","affiliation":[{"name":"Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06126 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Cassone","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita\u0300, 00186 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Vecchiarelli","sequence":"additional","affiliation":[{"name":"Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06126 Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01138475"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(94)90056-6"},{"key":"e_1_3_2_4_2","first-page":"505","volume":"131","year":"1985","unstructured":"Baccarini, M., A. 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