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In the present study, the abilities of seven recombinant putative leptospiral outer membrane proteins to interact with C4BP were evaluated. The protein encoded by LIC11947 interacted with this human complement regulator in a dose-dependent manner. The cofactor activity of C4BP bound to immobilized recombinant LIC11947 (rLIC11947) was confirmed by detecting factor I-mediated cleavage of C4b. rLIC11947 was therefore named LcpA (for<jats:underline>l<\/jats:underline>eptospiral<jats:underline>c<\/jats:underline>omplement regulator-acquiring<jats:underline>p<\/jats:underline>rotein<jats:underline>A<\/jats:underline>). LcpA was shown to be an outer membrane protein by using immunoelectron microscopy, cell surface proteolysis, and Triton X-114 fractionation. The gene coding for LcpA is conserved among pathogenic leptospiral strains. This is the first characterization of a<jats:italic>Leptospira<\/jats:italic>surface protein that binds to the human complement regulator C4BP in a manner that allows this important regulator to control complement system activation mediated either by the classical pathway or by the lectin pathway. This newly identified protein may play a role in immune evasion by<jats:italic>Leptospira<\/jats:italic>spp. and may therefore represent a target for the development of a human vaccine against leptospirosis.<\/jats:p>","DOI":"10.1128\/iai.00279-10","type":"journal-article","created":{"date-parts":[[2010,4,20]],"date-time":"2010-04-20T02:25:59Z","timestamp":1271730359000},"page":"3207-3216","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Functional Characterization of LcpA, a Surface-Exposed Protein of<i>Leptospira<\/i>spp. That Binds the Human Complement Regulator C4BP"],"prefix":"10.1128","volume":"78","author":[{"given":"Angela S.","family":"Barbosa","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Bacteriologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denize","family":"Monaris","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Bacteriologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Bacteriologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenaide M.","family":"Morais","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterina\u0301ria e Zootecnia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u0301lvio A.","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterina\u0301ria e Zootecnia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurora M.","family":"Cianciarullo","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Gene\u0301tica, Instituto Butantan, Sa\u0303o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Isaac","sequence":"additional","affiliation":[{"name":"Departamento de Imunologia, Instituto de Cie\u0302ncias Biome\u0301dicas, Universidade de Sa\u0303o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A. 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