{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:27:24Z","timestamp":1772162844133,"version":"3.50.1"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2018,6,21]],"date-time":"2018-06-21T00:00:00Z","timestamp":1529539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000272","name":"DH | National Institute for Health Research","doi-asserted-by":"publisher","award":["4RO1-AIO73558"],"award-info":[{"award-number":["4RO1-AIO73558"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"DH | National Institute for Health Research","doi-asserted-by":"publisher","award":["F31-AI115931"],"award-info":[{"award-number":["F31-AI115931"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    spp. are intracellular pathogenic bacteria remarkable in their ability to escape immune surveillance and therefore inflict a state of chronic disease within the host. To enable further immune response studies,\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    was engineered to express the well-characterized chicken ovalbumin (OVA). Surprisingly, we found that CD8 T cells bearing T cell receptors (TCR) nominally specific for the OVA peptide SIINFEKL (OT-1) reacted to parental\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    -infected targets as well as OVA-expressing\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    variants in cytotoxicity assays. Furthermore, splenocytes from\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    -immunized mice produced gamma interferon (IFN-\u03b3) and exhibited cytotoxicity in response to SIINFEKL-pulsed target cells.To determine if the SIINFEKL-reactive OT-1 TCR could be cross-reacting to\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    peptides, we searched the\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    proteome using an algorithm to generate a list of near-neighbor nonamer peptides that would bind to H2K\n                    <jats:sup>b<\/jats:sup>\n                    . Selecting five\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    peptide candidates, along with controls, we verified that several of these peptides mimicked SIINFEKL, resulting in T cell activation through the \u201cSIINFEKL-specific\u201d TCR. Activation was dependent on peptide concentration as well as sequence. Our results underscore the complexity and ubiquity of cross-reactivity in T cell recognition. This cross-reactivity may enable microbes such as\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    to escape immune surveillance by presenting peptides similar to those of the host and may also lead to the activation of autoreactive T cells.\n                  <\/jats:p>","DOI":"10.1128\/iai.00281-18","type":"journal-article","created":{"date-parts":[[2018,5,3]],"date-time":"2018-05-03T05:18:53Z","timestamp":1525324733000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Brucella Peptide Cross-Reactive Major Histocompatibility Complex Class I Presentation Activates SIINFEKL-Specific T Cell Receptor-Expressing T Cells"],"prefix":"10.1128","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1343-0977","authenticated-orcid":false,"given":"Jerome S.","family":"Harms","sequence":"first","affiliation":[{"name":"Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"},{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mike","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"},{"name":"Cellular and Molecular Pathology Training Program, School of Medicine and Public Health, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cherisse","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary A.","family":"Splitter","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Jane","family":"Homan","sequence":"additional","affiliation":[{"name":"ioGenetics LLC, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert D.","family":"Bremel","sequence":"additional","affiliation":[{"name":"ioGenetics LLC, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith A.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"},{"name":"Cellular and Molecular Pathology Training Program, School of Medicine and Public Health, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v26.i5.30"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034925"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01184-15"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.32.1.2190"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00222-5"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/686919"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250922"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2001.tb00536.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2000.00889.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90169-4"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/369750a0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.6.3695"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.1.420"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.11.5737"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.9.5341"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830211136"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301335"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00531-16"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3109\/08830185.2015.1018415"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.53.4.475"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/08830180902978120"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.305"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070115"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.13.6020"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8171333"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01905-06"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2009.12.004"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425132"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.2925-2936.2006"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2005.00329.x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-652-8_2"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-102"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003785"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000110"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v32.i4.50"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2003.11.004"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2001.00994.x"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3279"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2003.11.003"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.739"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115658"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(01)00298-9"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5441.958"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(98)01299-7"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2003.11.022"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00538"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000394"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867324666170508110222"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i22.3964"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2017.00216"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.04.006"},{"key":"e_1_3_3_53_2","first-page":"865","article-title":"The link between Proteus mirabilis, environmental factors and autoantibodies in rheumatoid arthritis","volume":"35","author":"Rashid T","year":"2017","unstructured":"Rashid T, Ebringer A, Wilson C. 2017. The link between Proteus mirabilis, environmental factors and autoantibodies in rheumatoid arthritis. Clin Exp Rheumatol 35:865\u2013871.","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-016-3171-7"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1P249"},{"key":"e_1_3_3_56_2","first-page":"S12","article-title":"Bacterial triggers and autoimmune rheumatic diseases","volume":"26","author":"Girschick HJ","year":"2008","unstructured":"Girschick HJ, Guilherme L, Inman RD, Latsch K, Rihl M, Sherer Y, Shoenfeld Y, Zeidler H, Arienti S, Doria A. 2008. Bacterial triggers and autoimmune rheumatic diseases. Clin Exp Rheumatol 26:S12\u2013S17.","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_3_3_57_2","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1111\/j.1742-1241.2003.tb10558.x","article-title":"Autoimmune hepatitis caused by Brucella: causal or a mere association?","volume":"57","author":"Justin S","year":"2003","unstructured":"Justin S, Gazzard B. 2003. Autoimmune hepatitis caused by Brucella: causal or a mere association? Int J Clin Pract 57:565\u2013566.","journal-title":"Int J Clin Pract"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00239-0"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.2741\/s341"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1568"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1139"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003937"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1186\/1745-7580-6-8"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026711"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00281-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00281-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T07:32:44Z","timestamp":1693639964000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00281-18"}},"subtitle":[],"editor":[{"given":"Guy H.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":63,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1128\/IAI.00281-18"],"URL":"https:\/\/doi.org\/10.1128\/iai.00281-18","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/301465","asserted-by":"object"}]},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"2018-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00281-18"}}