{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:01:55Z","timestamp":1791187315713,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            bacteriophage was used to identify a phage-resistant Tn\n            <jats:italic>917<\/jats:italic>\n            insertion mutant of the mouse-virulent listerial strain F6214-1. The mutant was attenuated when it was inoculated orally into female A\/J mice and failed to replicate efficiently in cultured mouse enterocytes. Phage binding studies indicated that the mutant had a cell surface alteration that precluded phage attachment. All phenotypes associated with the mutation could be complemented in\n            <jats:italic>trans<\/jats:italic>\n            by a single open reading frame (ORF) that corresponded to the ORF interrupted by the Tn\n            <jats:italic>917<\/jats:italic>\n            insertion. The complementation effected was, in all cases, at a level indistinguishable from that of the parent. The Tn\n            <jats:italic>917<\/jats:italic>\n            insertion interrupted a gene that is predicted to encode a group 2 glycosyl transferase (provisionally designated\n            <jats:italic>glcV<\/jats:italic>\n            ). A similar\n            <jats:italic>glcV<\/jats:italic>\n            gene is present in\n            <jats:italic>Listeria welshimeri<\/jats:italic>\n            and\n            <jats:italic>Listeria innocua<\/jats:italic>\n            and in some serotypes of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            . We speculate that the loss of the\n            <jats:italic>glcV<\/jats:italic>\n            product results in a defective phage receptor and that this alteration coincidentally influences a feature of the normal host-pathogen interaction required for virulence. Interestingly, the\n            <jats:italic>glcV<\/jats:italic>\n            lesion, while preventing phage attachment, did not alter the mutant's ability to bind to cultured mouse enterocyte monolayers. Rather, the mutation appeared to alter a subsequent step in intracellular replication measured by a reduction in plaque-forming efficiency and plaque size. In vivo, the mutant was undetectable in the liver and spleen 48 h after oral inoculation. The mutation is significant in part because it is one of the few that produce attenuation when the mutant is delivered orally.\n          <\/jats:p>","DOI":"10.1128\/iai.00283-08","type":"journal-article","created":{"date-parts":[[2008,6,17]],"date-time":"2008-06-17T00:45:09Z","timestamp":1213663509000},"page":"4046-4054","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["A\n            <i>Listeria monocytogenes<\/i>\n            Mutant Defective in Bacteriophage Attachment Is Attenuated in Orally Inoculated Mice and Impaired in Enterocyte Intracellular Growth"],"prefix":"10.1128","volume":"76","author":[{"given":"Patricia A.","family":"Spears","sequence":"first","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Mitsu","family":"Suyemoto","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Palermo","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Horton","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terri S.","family":"Hamrick","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Pharmacy, Campbell University, Buies Creek, North Carolina 27560"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward A.","family":"Havell","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Orndorff","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5953-5957.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2054-2065.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00039-06"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609672104"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.11890"},{"key":"e_1_3_2_7_2","first-page":"341","volume":"12","year":"1936","unstructured":"Burn, C. 1936. 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