{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T19:00:16Z","timestamp":1761418816950},"reference-count":33,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n A comprehensive Tn\n phoA<\/jats:italic>\n mutant library was constructed in\n Yersinia pestis<\/jats:named-content>\n KIM6 to identify surface proteins involved in\n Y. pestis<\/jats:named-content>\n host cell invasion and bacterial virulence. Insertion site analysis of the library repeatedly identified a 9,042-bp chromosomal gene (YPO3944), intimin\/invasin-like protein (Ilp), similar to the Gram-negative intimin\/invasin family of surface proteins. Deletion mutants of\n ilp<\/jats:italic>\n were generated in\n Y. pestis<\/jats:named-content>\n strains KIM5(pCD1\n +<\/jats:sup>\n ) Pgm\n \u2212<\/jats:sup>\n (pigmentation negative)\/, KIM6(pCD1\n \u2212<\/jats:sup>\n ) Pgm\n +<\/jats:sup>\n , and CO92. Comparative analyses were done with the deletions and the parental wild type for bacterial adhesion to and internalization by HEp-2 cells\n in vitro<\/jats:italic>\n , infectivity and maintenance in the flea vector, and lethality in murine models of systemic and pneumonic plague. Deletion of\n ilp<\/jats:italic>\n had no effect on bacterial blockage of flea blood feeding or colonization. The\n Y. pestis<\/jats:named-content>\n KIM5 \u0394\n ilp<\/jats:italic>\n strain had reduced adhesion to and internalization by HEp-2 cells compared to the parental wild-type strain (\n P<\/jats:italic>\n < 0.05). Following intravenous challenge with\n Y. pestis<\/jats:named-content>\n KIM5 \u0394\n ilp<\/jats:italic>\n , mice had a delayed time to death and reduced dissemination to the lungs, livers, and kidneys as monitored by\n in vivo<\/jats:italic>\n imaging using a\n lux<\/jats:italic>\n reporter system (\n in vivo<\/jats:italic>\n imaging system [IVIS]) and bacterial counts. Intranasal challenge in mice with\n Y. pestis<\/jats:named-content>\n CO92 \u0394\n ilp<\/jats:italic>\n had a 55-fold increase in the 50% lethal dose ([LD\n 50<\/jats:sub>\n ] 1.64 \u00d7 10\n 4<\/jats:sup>\n CFU) compared to the parental wild-type strain LD\n 50<\/jats:sub>\n (2.98 \u00d7 10\n 2<\/jats:sup>\n CFU). These findings identified Ilp as a novel virulence factor of\n Y. pestis<\/jats:named-content>\n .\n <\/jats:p>","DOI":"10.1128\/iai.00294-12","type":"journal-article","created":{"date-parts":[[2012,7,31]],"date-time":"2012-07-31T07:45:32Z","timestamp":1343720732000},"page":"3559-3569","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Role of a New Intimin\/Invasin-Like Protein in Yersinia pestis Virulence"],"prefix":"10.1128","volume":"80","author":[{"given":"Keun Seok","family":"Seo","sequence":"first","affiliation":[{"name":"Departments of Basic Sciences, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Wan","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Food Science, University of Idaho, Moscow, Idaho, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joo Youn","family":"Park","sequence":"additional","affiliation":[{"name":"Departments of Basic Sciences, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Austin K.","family":"Viall","sequence":"additional","affiliation":[{"name":"School of Food Science, University of Idaho, Moscow, Idaho, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott S.","family":"Minnich","sequence":"additional","affiliation":[{"name":"School of Food Science, University of Idaho, Moscow, Idaho, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold N.","family":"Rohde","sequence":"additional","affiliation":[{"name":"School of Food Science, University of Idaho, Moscow, Idaho, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darren R.","family":"Schnider","sequence":"additional","affiliation":[{"name":"School of Food Science, University of Idaho, Moscow, Idaho, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Yong","family":"Lim","sequence":"additional","affiliation":[{"name":"School of Food Science, University of Idaho, Moscow, Idaho, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon Bae","family":"Hong","sequence":"additional","affiliation":[{"name":"School of Food Science, University of Idaho, Moscow, Idaho, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. 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