{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T15:29:31Z","timestamp":1793114971265,"version":"4.5.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            utilizes the Dot\/Icm type IV translocation system to proliferate within a vacuole in a wide variety of natural amoebal hosts and in alveolar macrophages of the human accidental host. Although\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            utilizes host amino acids as the main sources of carbon and energy, it is not known whether\n            <jats:italic>de novo<\/jats:italic>\n            synthesis of amino acids by intravacuolar\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            contributes to its nutrition. The\n            <jats:italic>aroB<\/jats:italic>\n            and\n            <jats:italic>aroE<\/jats:italic>\n            genes encode enzymes for the shikimate pathway that generates the aromatic amino acids Phe, Trp, and Tyr. Here we show the\n            <jats:italic>aroB<\/jats:italic>\n            and\n            <jats:italic>aroE<\/jats:italic>\n            mutants of\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            to be defective in growth in human monocyte-derived macrophages (hMDMs) but not in\n            <jats:named-content content-type=\"genus-species\">Acanthamoeba<\/jats:named-content>\n            spp. The\n            <jats:italic>aroB<\/jats:italic>\n            and\n            <jats:italic>aroE<\/jats:italic>\n            mutants are severely attenuated in intrapulmonary proliferation in the A\/J mouse model of Legionnaires' disease, and the defect is fully complemented by the respective wild-type alleles. The two mutants grow normally in rich media but do not grow in defined media lacking aromatic amino acids, and the growth defect is rescued by inclusion of the aromatic amino acids, which are essential for production of the pyomelanin pigment. Interestingly, supplementation of infected hMDMs with the three aromatic amino acids or with Trp alone rescues the intramacrophage defect of the\n            <jats:italic>aroE<\/jats:italic>\n            but not the\n            <jats:italic>aroB<\/jats:italic>\n            mutant. Therefore, the shikimate pathway of\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            is differentially required for optimal growth within human macrophages, which are auxotrophic for Trp and Phe, but is dispensable for growth within the\n            <jats:named-content content-type=\"genus-species\">Acanthamoeba<\/jats:named-content>\n            spp. that synthesize the aromatic amino acids.\n          <\/jats:p>","DOI":"10.1128\/iai.00294-15","type":"journal-article","created":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T01:42:15Z","timestamp":1428370935000},"page":"2487-2495","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Selective Requirement of the Shikimate Pathway of Legionella pneumophila for Intravacuolar Growth within Human Macrophages but Not within Acanthamoeba"],"prefix":"10.1128","volume":"83","author":[{"given":"Snake C.","family":"Jones","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology and Center for Predictive Medicine, College of Medicine, University of Louisville, Louisville, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher T. 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