{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T10:59:07Z","timestamp":1792407547541,"version":"4.4.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The regulation of cellular processes by eukaryote-like serine\/threonine kinases is widespread in bacteria. In the last 2 years, several studies have examined the role of serine\/threonine kinases in\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            on cell wall metabolism, autolysis, and virulence, mostly in\n            <jats:italic>S. aureus<\/jats:italic>\n            laboratory isolates in the 8325-4 lineage. In this study, we showed that the\n            <jats:italic>pknB<\/jats:italic>\n            gene (also called\n            <jats:italic>stk1<\/jats:italic>\n            ) of methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            (MRSA) strain COL and the community-acquired MRSA (CA-MRSA) strain USA300 is involved in cell wall metabolism, with the\n            <jats:italic>pknB<\/jats:italic>\n            mutant exhibiting enhanced sensitivity to \u03b2-lactam antibiotics but not to other classes of antibiotics, including aminoglycosides, ciprofloxacin, bactrim, and other types of cell wall-active agents (e.g., vancomycin and bacitracin). Additionally, the\n            <jats:italic>pknB<\/jats:italic>\n            mutant of USA300 was found to be more resistant to Triton X-100-induced autolysis and also to lysis by lysostaphin. We also showed that\n            <jats:italic>pknB<\/jats:italic>\n            is a positive regulator of\n            <jats:italic>sigB<\/jats:italic>\n            activity, resulting in compromise in its response to heat and oxidative stresses. In association with reduced\n            <jats:italic>sigB<\/jats:italic>\n            activity, the expression levels of RNAII and RNAIII of\n            <jats:italic>agr<\/jats:italic>\n            and the downstream effector\n            <jats:italic>hla<\/jats:italic>\n            are upregulated while\n            <jats:italic>spa<\/jats:italic>\n            expression is downmodulated in the\n            <jats:italic>pknB<\/jats:italic>\n            mutant compared to the level in the parent. Consistent with an enhanced\n            <jats:italic>agr<\/jats:italic>\n            response\n            <jats:italic>in vitro<\/jats:italic>\n            , virulence studies of the\n            <jats:italic>pknB<\/jats:italic>\n            mutant of USA300 in a murine cutaneous model of infection showed that the mutant was more virulent than the parental strain. Collectively, our results have linked the\n            <jats:italic>pknB<\/jats:italic>\n            gene in CA-MRSA to antibiotic resistance,\n            <jats:italic>sigB<\/jats:italic>\n            activity, and virulence and have highlighted important differences in\n            <jats:italic>pknB<\/jats:italic>\n            phenotypes (virulence and\n            <jats:italic>sigB<\/jats:italic>\n            activity) between laboratory isolates and the prototypic CA-MRSA strain USA300.\n          <\/jats:p>","DOI":"10.1128\/iai.00296-10","type":"journal-article","created":{"date-parts":[[2010,6,15]],"date-time":"2010-06-15T03:51:01Z","timestamp":1276573861000},"page":"3637-3646","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":95,"title":["Role of PknB Kinase in Antibiotic Resistance and Virulence in Community-Acquired Methicillin-Resistant\n            <i>Staphylococcus aureus<\/i>\n            Strain USA300"],"prefix":"10.1128","volume":"78","author":[{"given":"Sandeep","family":"Tamber","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Schwartzman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambrose L.","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.11.6887-6891.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01734-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(99)01681-5"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7851-7857.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01499-08"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.13.4085-4099.2004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.3.1331-1337.1999"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01826-07"},{"key":"e_1_3_2_10_2","unstructured":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically 2006"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01813-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.01930.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00117-09"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02832.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.14.3955-3964.2000"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.293.24.3012"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.19.5457-5467.2002"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4119-4126.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.298.15.1763"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0900743106"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/595982"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00049-08"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.1.33"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2009.08.016"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.22.6525-6531.2001"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1139\/w04-017"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00859-08"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1086\/595740"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01068-08"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01087-08"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(02)02164-3"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00296-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00296-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:46:41Z","timestamp":1646473601000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00296-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["10.1128\/IAI.00296-10"],"URL":"https:\/\/doi.org\/10.1128\/iai.00296-10","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,8]]},"assertion":[{"value":"2010-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-05-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}