{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T01:28:41Z","timestamp":1773192521956,"version":"3.50.1"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI059062"],"award-info":[{"award-number":["AI059062"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            survives in nature through a complex tick-mammalian life cycle. During its transit between ticks and mammalian hosts,\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            must dramatically alter its outer surface profile in order to interact with and adapt to these two diverse niches. It has been established that the regulator BosR (BB0647) in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            plays important roles in modulating borrelial host adaptation. However, to date, how\n            <jats:italic>bosR<\/jats:italic>\n            expression itself is controlled in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            remains largely unknown. Previously, it has been shown that DNA sequences upstream of BosR harbor multiple sites for the binding of recombinant BosR, suggesting that BosR may influence its own expression in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            . However, direct experimental evidence supporting this putative autoregulation of BosR has been lacking. Here, we investigated the expression of\n            <jats:italic>bosR<\/jats:italic>\n            throughout the tick-mammal life cycle of\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            via quantitative reverse transcription (RT)-PCR analyses. Our data indicated that\n            <jats:italic>bosR<\/jats:italic>\n            is expressed not only during mouse infection, but also during the tick acquisition, intermolt, and transmission phases. Further investigation revealed that\n            <jats:italic>bosR<\/jats:italic>\n            expression in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            is influenced by environmental stimuli, such as temperature shift and pH change. By employing luciferase reporter assays, we also identified two promoters potentially driving\n            <jats:italic>bosR<\/jats:italic>\n            transcription. Our study offers strong support for the long-postulated function of BosR as an autoregulator in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00297-16","type":"journal-article","created":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T03:05:52Z","timestamp":1466564752000},"page":"2566-2574","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Evidence that BosR (BB0647) Is a Positive Autoregulator in Borrelia burgdorferi"],"prefix":"10.1128","volume":"84","author":[{"given":"Zhiming","family":"Ouyang","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianli","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael V.","family":"Norgard","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2015.02.010"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7043737"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303313081301"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2714"},{"key":"e_1_3_2_6_2","volume-title":"Borrelia: molecular biology, host interaction and pathogenesis","author":"Samuels DS","year":"2010","unstructured":"Samuels DS, Radolf JD. 2010. 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