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To expand our knowledge of host\u2013parasite lipid interactions, we studied\n              <jats:italic>T. gondii<\/jats:italic>\n              in intestinal epithelial cells, the first site of host\u2013parasite contact following oral infection and the exclusive site of parasite sexual development in feline hosts. We found that highly metabolic Caco-2 cells are permissive to\n              <jats:italic>T. gondii<\/jats:italic>\n              growth even when treated with high levels of linoleic acid (LA), a polyunsaturated fatty acid (PUFA) that kills parasites in HFFs. Caco-2 cells appear to sequester LA away from the parasite, preventing membrane disruptions and lipotoxicity that characterize LA-induced parasite death in HFFs. Our work is an important step toward understanding host\u2013parasite interactions in feline intestinal epithelial cells, an understudied but important cell type in the\n              <jats:italic>T. gondii<\/jats:italic>\n              life cycle.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00299-24","type":"journal-article","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T13:00:44Z","timestamp":1724850044000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Host cell-specific metabolism of linoleic acid controls\n            <i>Toxoplasma gondii<\/i>\n            growth in cell culture"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6602-6399","authenticated-orcid":false,"given":"Nicole D.","family":"Hryckowian","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos J.","family":"Ram\u00edrez-Flores","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin","family":"Zinda","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Chul","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin T.","family":"Kelty","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9362-8088","authenticated-orcid":false,"given":"Laura J.","family":"Knoll","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197405162902003"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091014-104100"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1972.tb03431.x"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.11.024"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.608291"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2006"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0000000000000084"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-809954-4.00046-3"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1220272110"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01126-12"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.1990.10720403"},{"key":"e_1_3_4_13_2","first-page":"75","volume-title":"New comprehensive biochemistry","author":"Schulz H.","year":"1996","unstructured":"Schulz H. 1996. 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