{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:29:35Z","timestamp":1790389775056,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | NIH | NIAID | Division of Intramural Research, National Institute of Allergy and Infectious Diseases","award":["R21AI166889"],"award-info":[{"award-number":["R21AI166889"]}]},{"name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","award":["R01AI132460"],"award-info":[{"award-number":["R01AI132460"]}]},{"name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","award":["T32AI138954"],"award-info":[{"award-number":["T32AI138954"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2023,12,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    is a Gram-negative bacterium that causes a variety of human diseases, ranging from pneumonia to urinary tract infections and invasive diseases. The emergence of\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    strains that are resistant to multiple antibiotics has made treatment more complex and led to\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    becoming a global health threat. Addressing this threat necessitates the development of new therapeutic strategies to combat this pathogen, including strategies to overcome antimicrobial resistance and therapeutics for novel targets such as antivirulence. Here, we investigated the function of TolC, an outer membrane protein essential for the function of tripartite transporters, in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    . Mutation of\n                    <jats:italic>tolC<\/jats:italic>\n                    rendered\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    hypersensitive to multiple antibiotics. Moreover, the\n                    <jats:italic>tolC<\/jats:italic>\n                    mutation impaired capsule production and affected the expression of key capsule biosynthetic genes, indicating a regulatory role for TolC in capsule biosynthesis. Additionally, TolC was essential for growth under iron-limiting conditions, suggesting its involvement in iron acquisition. The\n                    <jats:italic>tolC<\/jats:italic>\n                    mutant exhibited increased adherence to human enterocytes and enhanced serum sensitivity. In the\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    infection model, the\n                    <jats:italic>tolC<\/jats:italic>\n                    mutant displayed reduced virulence compared to the wild type. Our findings highlight the pleiotropic role of TolC in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    pathobiology, influencing antimicrobial resistance, capsule production, iron homeostasis, adherence to host cells, and virulence. Understanding the multifaceted role of TolC in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    may guide the development of new therapeutic strategies against this pathogen.\u2003\n                  <\/jats:p>","DOI":"10.1128\/iai.00303-23","type":"journal-article","created":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T09:00:49Z","timestamp":1700470849000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["<i>Klebsiella pneumoniae<\/i>\n                    TolC contributes to antimicrobial resistance, exopolysaccharide production, and virulence"],"prefix":"10.1128","volume":"91","author":[{"given":"X. Renee","family":"Bina","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuding","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Budnick","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia E.","family":"Van Allen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, 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