{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:43:36Z","timestamp":1790257416847,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The<jats:italic>YPS3<\/jats:italic>gene of<jats:italic>Histoplasma capsulatum<\/jats:italic>encodes a protein that is both surface localized in the cell wall of<jats:italic>H. capsulatum<\/jats:italic>and released into the culture medium. This protein is produced only during the pathogenic yeast phase of infection and is also expressed differentially in<jats:italic>H. capsulatum<\/jats:italic>strains of different virulence levels. In this study, we silenced the<jats:italic>YPS3<\/jats:italic>transcript by using an interfering-RNA strategy and examined the silenced mutants for phenotypic differences in vitro and during infection. The mutants showed no growth defect during in vitro culture in a defined medium at 37\u00b0C and appeared to have normal virulence in a RAW 264.7 murine macrophage-like cell line. In a C57BL\/6 mouse model of infection, however, the mutants caused significantly decreased fungal burdens, particularly in the peripheral phagocyte-rich tissues of livers and spleens. This defect in organ colonization was evident within 3 days of infection; however, it appeared to be exacerbated at later time points.<\/jats:p>","DOI":"10.1128\/iai.00304-07","type":"journal-article","created":{"date-parts":[[2007,4,3]],"date-time":"2007-04-03T00:44:08Z","timestamp":1175561048000},"page":"2811-2817","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["RNA Interference-Mediated Silencing of the<i>YPS3<\/i>Gene of<i>Histoplasma capsulatum<\/i>Reveals Virulence Defects"],"prefix":"10.1128","volume":"75","author":[{"given":"Megan L.","family":"Bohse","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon P.","family":"Woods","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"6072","DOI":"10.4049\/jimmunol.160.12.6072","volume":"160","year":"1998","unstructured":"Allendoerfer, R., and G. 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