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In\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            , CpxRA senses and responds to envelope stress; CpxA is a sensor kinase\/phosphatase for CpxR, a response regulator. When a\n            <jats:italic>cpxA<\/jats:italic>\n            mutant is grown in medium containing glucose, CpxR is phosphorylated by acetyl phosphate but cannot be dephosphorylated, resulting in constitutive activation. Kandler and coworkers (J. L. Kandler, C. L. Holley, J. L. Reimche, V. Dhulipala, J. T. Balthazar, A. Muszy\u0144ski, R. W. Carlson, and W. M. Shafer, Antimicrob Agents Chemother 60:4690\u20134700, 2016,\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1128\/AAC.00823-16\">https:\/\/doi.org\/10.1128\/AAC.00823-16<\/jats:ext-link>\n            ) showed that MisR (CpxR) is required for the maintenance of membrane integrity and resistance to antimicrobial peptides, suggesting a role in gonococcal survival\n            <jats:italic>in vivo<\/jats:italic>\n            . Here, we evaluated the contributions of MisR and MisS (CpxA) to gonococcal infection in a murine model of cervicovaginal colonization and identified MisR-regulated genes using RNA sequencing (RNA-Seq). The deletion of\n            <jats:italic>misR<\/jats:italic>\n            or\n            <jats:italic>misS<\/jats:italic>\n            severely reduced the capacity of\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            to colonize mice or maintain infection over a 7-day period and reduced microbial fitness after exposure to heat shock. Compared to the wild type (WT), the inactivation of\n            <jats:italic>misR<\/jats:italic>\n            identified 157 differentially regulated genes, most of which encoded putative envelope proteins. The inactivation of\n            <jats:italic>misS<\/jats:italic>\n            identified 17 differentially regulated genes compared to the WT and 139 differentially regulated genes compared to the\n            <jats:italic>misR<\/jats:italic>\n            mutant, 111 of which overlapped those differentially expressed in the comparison of the WT versus the\n            <jats:italic>misR<\/jats:italic>\n            mutant. These data indicate that an intact MisRS system is required for gonococcal infection of mice. Provided the MisR is constitutively phosphorylated in the\n            <jats:italic>misS<\/jats:italic>\n            mutant, the data suggest that controlled but not constitutive activation is required for gonococcal infection in mice.\n          <\/jats:p>","DOI":"10.1128\/iai.00307-17","type":"journal-article","created":{"date-parts":[[2017,6,27]],"date-time":"2017-06-27T00:20:22Z","timestamp":1498522822000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Both MisR (CpxR) and MisS (CpxA) Are Required for Neisseria gonorrhoeae Infection in a Murine Model of Lower Genital Tract Infection"],"prefix":"10.1128","volume":"85","author":[{"given":"Dharanesh","family":"Gangaiah","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica L.","family":"Raterman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, F. 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