{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:02:06Z","timestamp":1791266526703,"version":"4.1.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI083241"],"award-info":[{"award-number":["AI083241"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Gram-positive bacterium\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            transitions from an environmental organism to an intracellular pathogen following its ingestion by susceptible mammalian hosts. Bacterial replication within the cytosol of infected cells requires activation of the central virulence regulator PrfA followed by a PrfA-dependent induction of secreted virulence factors. The PrfA-induced secreted chaperone PrsA2 and the chaperone\/protease HtrA contribute to the folding and stability of select proteins translocated across the bacterial membrane.\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            strains that lack both\n            <jats:italic>prsA2<\/jats:italic>\n            and\n            <jats:italic>htrA<\/jats:italic>\n            exhibit near-normal patterns of growth in broth culture but are severely attenuated\n            <jats:italic>in vivo<\/jats:italic>\n            . We hypothesized that, in the absence of PrsA2 and HtrA, the increase in PrfA-dependent protein secretion that occurs following bacterial entry into the cytosol results in misfolded proteins accumulating at the bacterial membrane with a subsequent reduction in intracellular bacterial viability. Consistent with this hypothesis, the introduction of a constitutively activated allele of\n            <jats:italic>prfA<\/jats:italic>\n            (\n            <jats:italic>prfA*<\/jats:italic>\n            ) into \u0394\n            <jats:italic>prsA2<\/jats:italic>\n            \u0394\n            <jats:italic>htrA<\/jats:italic>\n            strains was found to essentially inhibit bacterial growth at 37\u00b0C in broth culture. \u0394\n            <jats:italic>prsA2<\/jats:italic>\n            \u0394\n            <jats:italic>htrA<\/jats:italic>\n            strains were additionally found to be defective for cell invasion and vacuole escape in selected cell types, steps that precede full PrfA activation. These data establish the essential requirement for PrsA2 and HtrA in maintaining bacterial growth under conditions of PrfA activation. In addition, chaperone function is required for efficient bacterial invasion and rapid vacuole lysis within select host cell types, indicating roles for PrsA2\/HtrA prior to cytosolic PrfA activation and the subsequent induction of virulence factor secretion.\n          <\/jats:p>","DOI":"10.1128\/iai.00312-16","type":"journal-article","created":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T03:05:13Z","timestamp":1470107113000},"page":"3034-3046","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Secretion Chaperones PrsA2 and HtrA Are Required for Listeria monocytogenes Replication following Intracellular Induction of Virulence Factor Secretion"],"prefix":"10.1128","volume":"84","author":[{"given":"Jana K.","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology, University of Illinois at Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Illinois at Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00282-12"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-69.1.93"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.30.1.29-32.1975"},{"key":"e_1_3_3_5_2","first-page":"1357","article-title":"Multistate outbreak of listeriosis associated with Jensen Farms cantaloupe\u2013United States, August-September 2011","volume":"60","author":"Centers for Disease Control and Prevention","year":"2011","unstructured":"Centers for Disease Control and Prevention. 2011. Multistate outbreak of listeriosis associated with Jensen Farms cantaloupe\u2013United States, August-September 2011. MMWR Morb Mortal Wkly Rep 60:1357\u20131358.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/428575"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2171"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.01.005"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.007"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00886-06"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.2.5.17703"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394590-7.00007-5"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.1.219-230.1992"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb02118.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/345175a0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.11.2822-2829.1987"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90188-I"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.11890"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14029"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.5.1533-1540.1997"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2008.06.006"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.1917-1926.2005"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03534.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04052.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.18.6265-6276.2004"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00845-07"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1631545"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302322200"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2011.0210"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04535.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00532-10"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05307-11"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00280-09"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80743-6"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.10.007"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4241-4247.2005"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.4.1935-1943.2004"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.765-768.2006"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07665.x"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00504-15"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00013"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01643.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(55)90016-7"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/021063-0"},{"key":"e_1_3_3_45_2","first-page":"79","volume-title":"Plasmids: a practical approach","author":"Youngman P","year":"1987","unstructured":"Youngman P. 1987. Plasmid vectors recovering and exploiting Tn917 transposons in Bacillus and other Gram-positive bacteria, p 79\u2013103. In Hardy KG (ed), Plasmids: a practical approach. IRL Press, Oxford, United Kingdom."},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1844-1852.1999"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00604.x"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.14.5522"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00905-4"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.6.2537-2544.1993"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.4.1597"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1459"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.35.24567"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.11.4531-4534.1995"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02576.x"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2784-2790.2002"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.21.6840-6843.1999"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10872.x"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00016-09"},{"key":"e_1_3_3_61_2","first-page":"2005","article-title":"Adoptive transfer of immunity to Listeria monocytogenes","volume":"139","author":"Bishop DK","year":"1987","unstructured":"Bishop DK, Hinrichs DJ. 1987. Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements. J Immunol 139:2005\u20132009.","journal-title":"The influence of in vitro stimulation on lymphocyte subset requirements. J Immunol"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.12.5608-5613.1994"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00312-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00312-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:07:19Z","timestamp":1646474839000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00312-16"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":61,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1128\/IAI.00312-16"],"URL":"https:\/\/doi.org\/10.1128\/iai.00312-16","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"2016-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}