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The existing vaccines are not ideal for preventing\n            <jats:italic>H. pylori<\/jats:italic>\n            infection; thus, exploring highly effective adjuvants may improve the immunoprotective efficacy of\n            <jats:italic>H. pylori<\/jats:italic>\n            vaccines. In a previous study, we found that the outer membrane vesicles (OMVs), a type of nanoscale particle spontaneously produced by Gram-negative bacteria, could act as adjuvants to boost the immune responses to vaccine antigens. In this study, we explored the potential application of OMVs as delivery vectors for adjuvant development. We constructed recombinant OMVs containing eukaryotic expression plasmid of cytokines, including interleukin 17A or interferon-\u03b3, and evaluated their function as adjuvants in combination with inactivated whole-cell vaccine (WCV) or UreB as vaccine antigens. Our results showed that recombinant OMVs as adjuvants could induce stronger humoral and mucosal immune responses in mice than wild-type\n            <jats:italic>H. pylori<\/jats:italic>\n            OMVs and the cholera toxin (CT) adjuvant. Additionally, the recombinant OMVs significantly promoted Th1\/Th2\/Th17-type immune responses. Furthermore, the recombinant OMV adjuvant induced more potent clearance of\n            <jats:italic>H. pylori<\/jats:italic>\n            than CT and wild-type OMVs. Our findings suggest that the recombinant OMVs coupled with cytokines may become potent adjuvants for the development of novel and effective vaccines against\n            <jats:italic>H. pylori<\/jats:italic>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.00313-23","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T13:00:43Z","timestamp":1698411643000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Recombinant outer membrane vesicles delivering eukaryotic expression plasmid of cytokines act as enhanced adjuvants against\n            <i>Helicobacter pylori<\/i>\n            infection in mice"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8950-6133","authenticated-orcid":false,"given":"Qiong","family":"Liu","sequence":"first","affiliation":[{"name":"Center for Molecular Diagnosis and Precision Medicine, and The Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University , Nanchang, China"},{"name":"The Department of Medical Microbiology, School of Medicine, Nanchang University , Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biaoxian","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Molecular Diagnosis and Precision Medicine, and The Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University , Nanchang, China"},{"name":"The Department of Medical Microbiology, School of Medicine, Nanchang University , Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Lu","sequence":"additional","affiliation":[{"name":"Center for Molecular Diagnosis and Precision Medicine, and The Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University , Nanchang, China"},{"name":"The Department of Medical Microbiology, School of Medicine, Nanchang University , Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yejia","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Department of Medical Microbiology, School of Medicine, Nanchang University , Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinpan","family":"Shang","sequence":"additional","affiliation":[{"name":"The Department of Medical Microbiology, School of Medicine, Nanchang University , Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Li","sequence":"additional","affiliation":[{"name":"The Department of Medical Microbiology, School of Medicine, Nanchang University , Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Gong","sequence":"additional","affiliation":[{"name":"Center for Molecular Diagnosis and Precision Medicine, and The Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University , Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengsheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Molecular Diagnosis and Precision Medicine, and The Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University , Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v25.i37.5578"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-021-00449-x"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2018.1526680"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12807"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01003"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/5584_2019_370"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00583-2"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01115"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2021.1945450"},{"key":"e_1_3_4_11_2","first-page":"115","article-title":"Helicobacter pylori vaccine: mucosal adjuvant & delivery systems","volume":"130","author":"Sijun H","year":"2009","unstructured":"Sijun H , Yong X . 2009 . 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