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Its endemicity and ineffective treatment options emphasize its public health threat and highlight the need for a vaccine. Live attenuated vaccines are considered the most viable vaccine strategy for\n            <jats:named-content content-type=\"genus-species\">Burkholderia<\/jats:named-content>\n            , but single-gene-deletion mutants have not provided complete protection. In this study, we constructed the select-agent-excluded\n            <jats:named-content content-type=\"genus-species\">B. mallei<\/jats:named-content>\n            \u0394\n            <jats:italic>tonB<\/jats:italic>\n            \u0394\n            <jats:italic>hcp1<\/jats:italic>\n            (CLH001) vaccine strain and investigated its ability to protect against acute respiratory glanders. Here we show that CLH001 is attenuated, safe, and effective at protecting against lethal\n            <jats:named-content content-type=\"genus-species\">B. mallei<\/jats:named-content>\n            challenge. Intranasal administration of CLH001 to BALB\/c and NOD SCID gamma (NSG) mice resulted in complete survival without detectable colonization or abnormal organ histopathology. Additionally, BALB\/c mice intranasally immunized with CLH001 in a prime\/boost regimen were fully protected against lethal challenge with the\n            <jats:named-content content-type=\"genus-species\">B. mallei<\/jats:named-content>\n            <jats:italic>lux<\/jats:italic>\n            (CSM001) wild-type strain.\n          <\/jats:p>","DOI":"10.1128\/iai.00328-16","type":"journal-article","created":{"date-parts":[[2016,6,7]],"date-time":"2016-06-07T03:13:47Z","timestamp":1465269227000},"page":"2345-2354","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Burkholderia mallei CLH001 Attenuated Vaccine Strain Is Immunogenic and Protects against Acute Respiratory Glanders"],"prefix":"10.1128","volume":"84","author":[{"given":"Christopher L.","family":"Hatcher","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiffany M.","family":"Mott","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura A.","family":"Muruato","sequence":"additional","affiliation":[{"name":"Institute for Translational Sciences, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Sbrana","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo G.","family":"Torres","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Institute for Translational Sciences, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134030"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1865-1682.2012.01342.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-8-131"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3923\/jbs.2014.38.51"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-26-1-93"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200107263450404"},{"key":"e_1_3_2_8_2","first-page":"167","article-title":"Emergence and re-emergence of glanders in India: a description of outbreaks from 2006 to 2011","volume":"48","author":"Malik P","year":"2012","unstructured":"Malik P, Singha H, Khurana SK, Kumar R, Kumar S, Raut AA, Riyesh T, Vaid RK, Virmani N, Singh BK, Pathak SV, Parkale DD, Singh B, Pandey SB, Sharma TR, Chauhan BC, Awasthi V, Jain S, Singh RK. 2012. Emergence and re-emergence of glanders in India: a description of outbreaks from 2006 to 2011. Vet Ital 48:167\u2013178.","journal-title":"Vet Ital"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1512.090738"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003195"},{"key":"e_1_3_2_11_2","first-page":"121","volume-title":"Medical aspects of biological warfare","author":"Gregory CG","year":"2007","unstructured":"Gregory CG, Waag DM. 2007. Glanders, chapter 6, p 121\u2013146. In Dembek ZF (ed), Medical aspects of biological warfare. Borden Institute, Walter Reed Army Medical Center, Washington, DC."},{"key":"e_1_3_2_12_2","first-page":"2869","volume-title":"Principles and practice of infectious diseases","author":"Currie BJ","year":"2010","unstructured":"Currie BJ. 2010. Burkholderia pseudomallei and Burkholderia mallei: melioidosis and glanders, p 2869\u20132879. 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