{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:54:13Z","timestamp":1785430453135,"version":"3.56.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["309736\/2021-8"],"award-info":[{"award-number":["309736\/2021-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["311470\/2018-1"],"award-info":[{"award-number":["311470\/2018-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MCTI - Colombia","award":["885\/2020"],"award-info":[{"award-number":["885\/2020"]}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"crossref","award":["E-26\/200.997\/2022"],"award-info":[{"award-number":["E-26\/200.997\/2022"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,12,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Candida auris<\/jats:italic>\n                      is an emerging fungal pathogen recognized among the Centers for Disease Control and Prevention\u2019s urgent threats and designated a critical priority by the World Health Organization due to its global spread, high mortality rates, potential for pan-drug resistance, and its persistent transmission within healthcare settings. The clinical management of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      infections is further hindered by the lack of both rapid\/specific diagnosis and effective antifungals. These challenges emphasize the urgent need for alternative therapeutic strategies. In this study, we investigated the antifungal, immunomodulatory, and protective effects of engineered Lectin-Fc(IgG) fusion proteins against a fluconazole-resistant\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      strain. Specifically, Dectin-1-Fc(IgG2a), Dectin-1-Fc(IgG2b), and wheat germ agglutinin (WGA)-Fc(IgG2a) demonstrated dose-dependent binding to key fungal cell wall components, \u03b2-1,3-glucan and chitin, with Dectin-1-Fc(IgG2b) exhibiting the highest reactivity, followed by Dectin-1-Fc(IgG2a) and WGA-Fc(IgG2a).\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      , all constructs exhibited fungistatic activity and reduced the biofilm biomass and metabolism, with the Dectin-1-Fc(IgG) variants displaying the most potent effects. As opsonins, Lectin-Fc(IgG)s significantly enhanced the macrophage\u2013yeast association and macrophage-mediated killing of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      . In a systemic murine\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      infection model, a single therapeutic administration of Dectin-1-Fc(IgG2b) or WGA-Fc(IgG2a) conferred 100% protection, while Dectin-1-Fc(IgG2a) achieved &gt;80% protection, with all treated mice manifesting clinical improvement. Quantification of fungal burden at day 7 post-infection revealed at least a ~1 log reduction in colony-forming units in the spleen, kidney, and liver of Lectin-Fc(IgG)-treated animals. Cytokine profiling indicated a Th1-type-skewed immune response in Lectin-Fc(IgG)-treated mice. Collectively, these findings support the antifungal and immunotherapeutic potential of Lectin-Fc(IgG)s against\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      , offering a novel broad-spectrum strategy to overcome current therapeutic limitations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00329-25","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:01:49Z","timestamp":1761742909000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Lectin-Fc(IgG) fusion proteins exhibit antifungal activity against the emerging multidrug-resistant pathogen\n                    <i>Candida auris<\/i>"],"prefix":"10.1128","volume":"93","author":[{"given":"Susana Ruiz","family":"Mendoza","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rjhbb08","id-type":"ROR","asserted-by":"publisher"}],"name":"Laborat\u00f3rio de Bioqu\u00edmica e Imunologia das Micoses, Departamento de Microbiologia e Parasitologia, Instituto Biom\u00e9dico, Universidade Federal Fluminense","place":["Niter\u00f3i, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"P\u00f3s-Gradua\u00e7\u00e3o em Imunologia e Inflama\u00e7\u00e3o, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro","family":"Honorato","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Laborat\u00f3rio de Glicobiologia de Eucariotos, Instituto de Microbiologia Professor Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, 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An\u00e1lise multifatorial da atividade antif\u00fangica de prote\u00ednas-de fus\u00e3o lectina-fc(igg) contra glicanas da superf\u00edcie f\u00fangica. 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