{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:01:02Z","timestamp":1791403262955,"version":"4.3.3"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T00:00:00Z","timestamp":1707782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T00:00:00Z","timestamp":1707782400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","award":["RGPAS-2017-507827"],"award-info":[{"award-number":["RGPAS-2017-507827"]}]},{"DOI":"10.13039\/100014407","name":"Ministry of Advanced Education, Government of Alberta","doi-asserted-by":"crossref","award":["RCP-19-003-MIF"],"award-info":[{"award-number":["RCP-19-003-MIF"]}],"id":[{"id":"10.13039\/100014407","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100012236","name":"Alberta Agriculture and Forestry","doi-asserted-by":"crossref","award":["Results Driven Agriculture Research 2019F040R"],"award-info":[{"award-number":["Results Driven Agriculture Research 2019F040R"]}],"id":[{"id":"10.13039\/100012236","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100012236","name":"Alberta Agriculture and Forestry","doi-asserted-by":"crossref","award":["Results Driven Agriculture Research 2020F036R"],"award-info":[{"award-number":["Results Driven Agriculture Research 2020F036R"]}],"id":[{"id":"10.13039\/100012236","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2024,2,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Digital dermatitis (DD) is a skin disease in cattle characterized by painful inflammatory ulcerative lesions in the feet, mostly associated with local colonization by\n            <jats:italic>Treponema<\/jats:italic>\n            spp., including\n            <jats:italic>Treponema phagedenis<\/jats:italic>\n            . The reason why most DD lesions remain actively inflamed and progress to chronic conditions despite antibiotic treatment remains unknown. Herein, we show an abundant infiltration of proinflammatory (CD14\n            <jats:sup>high<\/jats:sup>\n            CD16\n            <jats:sup>low<\/jats:sup>\n            ) monocytes\/macrophages in active DD lesions, a skin response that was not mitigated by topical treatment with oxytetracycline. The associated bacterium,\n            <jats:italic>T. phagedenis<\/jats:italic>\n            , isolated from DD lesions in cattle, when injected subcutaneously into mice, induced abscesses with a local recruitment of Ly6G\n            <jats:sup>+<\/jats:sup>\n            neutrophils and proinflammatory (Ly6C\n            <jats:sup>high<\/jats:sup>\n            CCR2\n            <jats:sup>+<\/jats:sup>\n            ) monocytes\/macrophages, which appeared at infection onset (4 days post challenge) and persisted for at least 7 days post challenge. When exploring the ability of macrophages to regulate inflammation, we showed that bovine blood-derived macrophages challenged with live\n            <jats:italic>T. phagedenis<\/jats:italic>\n            or its structural components secreted IL-1\u03b2 via a mechanism dependent on the NLRP3 inflammasome. This study shows that proinflammatory characteristics of monocytes\/macrophages and neutrophils dominate active non-healing ulcerative lesions in active DD, thus likely impeding wound healing after antibiotic treatment.\n          <\/jats:p>","DOI":"10.1128\/iai.00342-23","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T14:02:16Z","timestamp":1704722536000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Proinflammatory CD14\n            <sup>high<\/sup>\n            CD16\n            <sup>low<\/sup>\n            monocytes\/macrophages prevail in\n            <i>Treponema phagedenis-<\/i>\n            associated bovine digital dermatitis"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4302-2381","authenticated-orcid":false,"given":"Priyoshi","family":"Lahiri","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0757-3915","authenticated-orcid":false,"given":"Rakel","family":"Arrazuria","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi Lin","family":"Tan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4824-9580","authenticated-orcid":false,"given":"Jeroen","family":"De Buck","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morley D.","family":"Hollenberg","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Orsel","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5450-7673","authenticated-orcid":false,"given":"Eduardo R.","family":"Cobo","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2003.01901.x"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1286-4579(02)01638-6"},{"key":"e_1_3_5_4_2","first-page":"203","article-title":"The biology, pathology, and immunology of syphilis","volume":"24","author":"Sell S","year":"1983","unstructured":"Sell S, Norris SJ. 1983. The biology, pathology, and immunology of syphilis. Int Rev Exp Pathol 24:203\u2013276.","journal-title":"Int Rev Exp Pathol"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani5030369"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00114-x"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.10.015"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02077-14"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.06.048"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01586"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.10.028"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00873-14"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.140.24.620"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-019-03082-y"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(00)74936-8"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0154481"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.147.18.503"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.163.19.574"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12729"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2017.02.002"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00993-10"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-018-0605-z"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.1998.tb00694.x"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.3.1180-1186.1999"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1053\/tvjl.1998.0276"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00304"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.16463"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.11.5455"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.12.3.505-511.1975"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1074-7613(03)00174-2"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01875"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.2174\/1871530321666211015091109"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.03.008"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00019-07"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1995.tb00174.x"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-015-0246-4"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-193"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.6.4131"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000017"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18071545"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071502"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.08.006"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.165"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2012.07.007"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.237.5.555"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26111-0"},{"key":"e_1_3_5_47_2","volume-title":"Medical Microbiology","author":"Radolf JD","year":"1996","unstructured":"Radolf JD. 1996. Treponema. In Baron S (ed), Medical Microbiology. The University of Texas Medical Branch at Galveston, Galveston (TX)."},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2011.06.005"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00342-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00342-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T23:33:09Z","timestamp":1715815989000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.00342-23"}},"subtitle":[],"editor":[{"given":"Victor J.","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,2,13]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2,13]]}},"alternative-id":["10.1128\/iai.00342-23"],"URL":"https:\/\/doi.org\/10.1128\/iai.00342-23","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,13]]},"assertion":[{"value":"2023-08-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00342-23"}}