{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:14:06Z","timestamp":1788455646290,"version":"build-2803163510"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100010822","name":"Chengdu Science and Technology Bureau","doi-asserted-by":"crossref","award":["No. 2022-YF05-00817-SN"],"award-info":[{"award-number":["No. 2022-YF05-00817-SN"]}],"id":[{"id":"10.13039\/501100010822","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2023,12,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Virulent\n            <jats:italic>Glaesserella parasuis<\/jats:italic>\n            may engender systemic infection characterized by fibrinous polyserositis and pneumonia.\n            <jats:italic>G. parasuis<\/jats:italic>\n            causes systemic disease through upper respiratory tract infection, but the mechanism has not been fully characterized. Tight junction (TJ) proteins maintain the integrity and impermeability of the epithelial barriers. In this work, we applied the recombinant cytolethal distending toxin (CDT) holotoxin and\n            <jats:italic>cdt<\/jats:italic>\n            -deficient mutants to assess whether CDT interacted with TJ proteins of airway tract cells. Our results indicated that CDT induced the TJ occludin (OCLN) expression in newborn pig tracheal epithelial cells within the first 3 hours of bacterial infection, followed by a significant decrease. Overexpression of OCLN in target cells made them more susceptible to\n            <jats:italic>G. parasuis<\/jats:italic>\n            adhesion, whereas ablation of OCLN expression by CRISPR\/Cas 9 gene editing technology in target cells decreased their susceptibility to bacterial adhesion. In addition, CDT treatment could upregulate the OCLN levels in the lung tissue of C57\/BL6 mice. In summary, highly virulent\n            <jats:italic>G. parasuis<\/jats:italic>\n            strain SC1401 stimulated the tight junction expression, resulting in higher bacterial adhesion to respiratory tract cells, and this process is closely related to CDT. Our results may provide novel insights into\n            <jats:italic>G. parasuis<\/jats:italic>\n            infection and CDT-mediated pathogenesis.\n          <\/jats:p>","DOI":"10.1128\/iai.00351-23","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T13:01:38Z","timestamp":1699275698000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Upregulation of occludin by cytolethal distending toxin facilitates\n            <i>Glaesserella parasuis<\/i>\n            adhesion to respiratory tract cells"],"prefix":"10.1128","volume":"91","author":[{"given":"Zhen","family":"Yang","sequence":"first","affiliation":[{"name":"Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research 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