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Our previous studies identified a\n                      <jats:italic toggle=\"yes\">Cryptococcus neoformans<\/jats:italic>\n                      mutant strain\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      \u0394 as a potential vaccine candidate. This strain contains deletion of the F-box protein Fbp1, a key subunit of the SCF E3 ligase complex necessary for ubiquitin-mediated proteolysis. Vaccination with heat-killed\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      \u0394 (HK-\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      ) can elicit an interferon gamma (IFN-\u03b3)-dependent Type 1 immune response and provide protection against\n                      <jats:italic toggle=\"yes\">C. neoformans<\/jats:italic>\n                      and its sibling species\n                      <jats:italic toggle=\"yes\">C. gattii<\/jats:italic>\n                      . However, we have yet to decipher the immunogenic factor(s) expressed by the\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      \u2206 mutant that are responsible for the induction of the protective immune response. In this study, we have identified that the capsule plays an important role in HK-\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      vaccine-mediated protection as acapsular HK-\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      cells showed diminished protection against wild-type challenge. Additionally, our studies have shown that Cytokine Inducing Glycoprotein 1 (Cig1), a GPI-anchored mannoprotein, is regulated by Fbp1 and contributes to the immunogenicity of HK-\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      . Deletion of Cig1 in the\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      \u0394 background resulted in decreased recruitment of antifungal effector T cells and diminished production of protective inflammatory cytokines by the host. Furthermore, loss of Cig1 in the\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      \u0394 mutant resulted in reduced protection in vaccination survival studies at lower vaccine inoculum doses compared to HK-\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      . In aggregate, these findings demonstrate Cig1 is an antigen contributing to the immunogenicity of HK-\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      that may be utilized to further optimize the HK-\n                      <jats:italic toggle=\"yes\">fbp1<\/jats:italic>\n                      fungal vaccine as a tool in the arsenal against invasive fungal infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00355-25","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T14:00:36Z","timestamp":1764338436000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Mannoprotein Cig1 contributes to the immunogenicity of a heat-killed F-box protein Fbp1\n                    <i>Cryptococcus neoformans<\/i>\n                    vaccine model"],"prefix":"10.1128","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3918-7971","authenticated-orcid":true,"given":"Samantha L.","family":"Avina","sequence":"first","affiliation":[{"name":"School of Graduate Studies at the Health Science Campus, New Jersey Medical School, Rutgers University Newark","place":["Newark, USA"]},{"name":"Public Health Research Institute, and Department of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers University","place":["Newark, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9276-095X","authenticated-orcid":true,"given":"Siddhi","family":"Pawar","sequence":"additional","affiliation":[{"name":"School of Graduate Studies at the Health Science Campus, New Jersey Medical School, Rutgers University Newark","place":["Newark, USA"]},{"name":"Public Health Research Institute, and Department of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers University","place":["Newark, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshni N.","family":"Kadam","sequence":"additional","affiliation":[{"name":"School of Graduate Studies at the Health Science Campus, New Jersey Medical School, Rutgers University Newark","place":["Newark, USA"]},{"name":"Public Health Research Institute, and Department of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers University","place":["Newark, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2173-5597","authenticated-orcid":true,"given":"Amariliz","family":"Rivera","sequence":"additional","affiliation":[{"name":"Center for Immunity and Inflammation, and Department of Pediatrics New Jersey Medical School, Rutgers University Newark","place":["Newark, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8196-1468","authenticated-orcid":true,"given":"Chaoyang","family":"Xue","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, and Department of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers University","place":["Newark, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00499-6"},{"key":"e_1_3_4_3_2","volume-title":"WHO fungal priority pathogens list to guide research, development and public health action","author":"World Health O","year":"2022","unstructured":"World Health O. 2022. 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