{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:36:07Z","timestamp":1785404167598,"version":"3.56.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI083241"],"award-info":[{"award-number":["AI083241"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI083241-03S1"],"award-info":[{"award-number":["AI083241-03S1"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["Al115954-01"],"award-info":[{"award-number":["Al115954-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,7,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Listeria monocytogenes<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">Lm<\/jats:italic>\n                      ) is a gram-positive pathogen that is widespread throughout the environment and known for its ability to infect mammalian hosts following the ingestion of contaminated food.\n                      <jats:italic toggle=\"yes\">Lm<\/jats:italic>\n                      uses a variety of mechanisms to survive challenging conditions experienced both during life in the outside environment and inside of the infected host. We recently described a novel two-component signaling system known as PieRS that regulates the secretion of the chaperone PrsA2, which is essential for bacterial virulence, as well as its related homolog PrsA1 and a variety of gene products of unknown function. Here, we examine the roles of the less characterized PieRS-regulated gene products and contrast their functions with PrsA2 in terms of bacterial survival under stress conditions and virulence in mice. Characterization of targeted in-frame deletion mutants of PieRS regulon members indicates\u2014in contrast to\n                      <jats:italic toggle=\"yes\">prsA2<\/jats:italic>\n                      mutants\u2014minimal contributions to stress survival and bacterial virulence. Modest contributions of select regulon members were associated with\n                      <jats:italic toggle=\"yes\">Lm<\/jats:italic>\n                      colonization of the gastrointestinal tract. The PieRS regulon thus consists of gene products that contribute to\n                      <jats:italic toggle=\"yes\">Lm<\/jats:italic>\n                      physiology in ways that are clearly distinct from PrsA2 and the chaperone\u2019s essential function for both stress survival and bacterial virulence.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00357-24","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T09:00:16Z","timestamp":1748336416000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the\n                    <i>Listeria monocytogenes<\/i>\n                    PieRS regulon distinguishes the function of the critical secretion chaperone PrsA2 from other regulon members"],"prefix":"10.1128","volume":"93","author":[{"given":"Xiomarie","family":"Alejandro-Navarreto","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02mpq6x41","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, University of Illinois at Chicago","place":["Chicago, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2061-319X","authenticated-orcid":false,"given":"Laty A.","family":"Cahoon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Sciences, University of Pittsburgh","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1322-3978","authenticated-orcid":true,"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02mpq6x41","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, University of Illinois at Chicago","place":["Chicago, USA"]},{"id":[{"id":"https:\/\/ror.org\/02mpq6x41","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pharmaceutical Sciences, University of Illinois at Chicago","place":["Chicago, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584-640.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2171"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2005.02559.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.afnr.2018.02.006"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408690490489378"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2019.02.007"},{"key":"e_1_3_2_8_2","unstructured":"Hoffmann S Maculloch B. 2015. 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