{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:23:53Z","timestamp":1785421433250,"version":"3.56.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2026,1,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Echinococcus granulosus sensu lato<\/jats:italic>\n                      antigen B (EgAgB) is a major parasite lipoprotein, produced by the hydatid and released at the host-parasite interface. Accumulating evidence supports that EgAgB may exert immunomodulatory effects on myeloid cells; however, the underlying molecular mechanisms remain poorly understood. We examined the impact of native EgAgB (nEgAgB) and recombinant EgAgB8\/1 (rEgAgB) on lipopolysaccharide (LPS)-induced activation of bone marrow-derived dendritic cells (BMDC) to help elucidate these mechanisms. Both immunoaffinity-purified nEgAgB or rEgAgB induced modest BMDC activation, indicated by the production of IL-6, IL-12p40, and nitric oxide, but not IFN-\u03b2. This activation was primarily attributed to LPS traces in EgAgB preparations since it was nearly abolished by a specific TLR4 inhibitor and in\n                      <jats:italic toggle=\"yes\">Tlr4<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      BMDC, while EgAgB binding to BMDC was TLR4-independent. Notably, both nEgAgB and rEgAgB inhibited LPS-induced cytokine and nitric oxide production and disrupted TLR4 dimerization and endocytosis. Competitive binding assays showed that EgAgB and human high-density lipoprotein (hHDL) similarly inhibited LPS binding to macrophages and BMDC; however, EgAgB more effectively suppressed LPS-induced cytokine secretion. Contrastingly, EgAgB did not modulate BMDC responses to lipoteichoic acid, unlike hHDL. Using dynamic light scattering and an ELISA-like assay, we demonstrated a higher potential of EgAgB to bind LPS than hHDL. Additionally, docking analyzes suggest the presence of a defined LPS-binding interface in EgAgB8\/1 subunit. Overall, these findings reveal a novel binding property of EgAgB, which enables it to act as an extracellular LPS scavenger, interfering with TLR4-mediated LPS recognition and downstream proinflammatory responses in myeloid cells.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00361-25","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T14:49:27Z","timestamp":1765896567000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["<i>Echinococcus granulosus<\/i>\n                    antigen B acts as an LPS-scavenging lipoprotein\n                    <i>in vitro,<\/i>\n                    preventing TLR4-mediated activation of dendritic cells"],"prefix":"10.1128","volume":"94","author":[{"given":"S.","family":"Lagos Magallanes","sequence":"first","affiliation":[{"name":"Unidad de Inmunolog\u00eda, Facultad de Ciencias, Instituto de Qu\u00edmica Biol\u00f3gica, Universidad de la Rep\u00fablica","place":["Montevideo, Uruguay"]},{"name":"\u00c1rea Inmunolog\u00eda, Departamento de Biociencias, Facultad de Qu\u00edmica, Universidad de la Republica Montevideo","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Beasley Lomazzi","sequence":"additional","affiliation":[{"name":"Unidad de Inmunolog\u00eda, Facultad de Ciencias, Instituto de Qu\u00edmica Biol\u00f3gica, Universidad de la Rep\u00fablica","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Zamarre\u00f1o","sequence":"additional","affiliation":[{"name":"Departamento de F\u00edsica, Instituto de F\u00edsica del Sur (IFISUR), Universidad Nacional del Sur (UNS), CONICET","place":["Bah\u00eda Blanca, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Carri\u00f3n","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04dpm2z73","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Inmunovirolog\u00eda-Unidad de Biof\u00edsica de Prote\u00ednas, Institut Pasteur","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Fl\u00f3","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04dpm2z73","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Inmunovirolog\u00eda-Unidad de Biof\u00edsica de Prote\u00ednas, Institut Pasteur","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Dutto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056tb7j80","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba","place":["C\u00f3rdoba, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Julve","sequence":"additional","affiliation":[{"name":"Research Group of Endocrinology, Diabetes and Nutrition, Institut de Recerca SANT PAU & Biomedical Research Networking Center for Diabetes and Associated Metabolic Diseases (CIBERDEM)","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Costabel","sequence":"additional","affiliation":[{"name":"Departamento de F\u00edsica, Instituto de F\u00edsica del Sur (IFISUR), Universidad Nacional del Sur (UNS), CONICET","place":["Bah\u00eda Blanca, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Maccioni","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056tb7j80","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba","place":["C\u00f3rdoba, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0696-4935","authenticated-orcid":false,"given":"A. 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