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Several differentially expressed proteins have been shown to be critical for the enzootic cycle of<jats:italic>B. burgdorferi<\/jats:italic>. In this study, we demonstrated that expression of the surface lipoprotein-encoding gene<jats:italic>bba07<\/jats:italic>is induced by an elevated temperature and a reduced pH during<jats:italic>in vitro<\/jats:italic>cultivation, as well as during nymphal tick feeding. Expression of<jats:italic>bba07<\/jats:italic>is regulated by the Rrp2-RpoN-RpoS pathway, a central regulatory network that is activated during nymphal feeding. By generating a<jats:italic>bba07<\/jats:italic>mutant of an infectious strain of<jats:italic>B. burgdorferi<\/jats:italic>, we demonstrated that although BBA07-deficient spirochetes were capable of infecting mice via needle inoculation and surviving in ticks, they were defective in infection of mammals via tick transmission. Complementation of the<jats:italic>bba07<\/jats:italic>mutant with a wild-type copy of<jats:italic>bba07<\/jats:italic>partially restored the transmission defect of the<jats:italic>bba07<\/jats:italic>mutant. Based on these findings, we concluded that the surface lipoprotein BBA07 is produced during tick feeding and facilitates optimal transmission of<jats:italic>B. burgdorferi<\/jats:italic>from the tick vector to a mammalian host.<\/jats:p>","DOI":"10.1128\/iai.00372-10","type":"journal-article","created":{"date-parts":[[2010,4,27]],"date-time":"2010-04-27T02:07:37Z","timestamp":1272334057000},"page":"2910-2918","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Role of the Surface Lipoprotein BBA07 in the Enzootic Cycle of<i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"78","author":[{"given":"Haijun","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana 46202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"He","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana 46202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane Jingyuan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana 46202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. 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