{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T21:53:01Z","timestamp":1789854781829,"version":"4.0.1"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In meningococcal septic shock, the dominant inducer of inflammation is lipopolysaccharide (LPS) in the outer membrane of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Neisseria meningitidis<\/jats:named-content>, while interleukin-10 (IL-10) is the principal anti-inflammatory cytokine. We have used microarrays and Ingenuity Pathway Analysis to study the global effects of IL-10 on gene expression induced by<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>, after exposure of human monocytes (<jats:italic>n<\/jats:italic>= 5) for 3 h to<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>(10<jats:sup>6<\/jats:sup>cells\/ml), recombinant human IL-10 (rhIL-10) (25 ng\/ml), and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>combined with rhIL-10.<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>and IL-10 differentially expressed 3,579 and 648 genes, respectively. IL-10 downregulated 125 genes which were upregulated by<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>, including NLRP3, the key molecule of the NLRP3 inflammasome. IL-10 also upregulated 270 genes which were downregulated by<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>, including members of the leukocyte immunuglobulin-like receptor (LIR) family. Fifty-three genes revealed a synergistically increased expression when<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>and IL-10 were combined. AIM2 (the principal molecule of the AIM2 inflammasome) was among these genes (fold change [FC], 18.3 versus 7.4 and 9.4 after stimulation by<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>and IL-10, respectively). We detected reduced concentrations (92% to 40%) of six cytokines (IL-1b, IL-6, IL-8, tumor necrosis factor alpha [TNF-\u03b1], macrophage inflammatory protein alpha [MIP-\u03b1], MIP-\u03b2) in the presence of IL-10, compared with concentrations with stimulation by<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>alone. Our data analysis of the effects of IL-10 on gene expression induced by<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>suggests that high plasma levels of IL-10 in meningococcal septic shock plasma may have a profound effect on a variety of functions and cellular processes in human monocytes, including cell-to-cell signaling, cellular movement, cellular development, antigen presentation, and cell death.<\/jats:p>","DOI":"10.1128\/iai.00386-12","type":"journal-article","created":{"date-parts":[[2012,9,11]],"date-time":"2012-09-11T05:49:10Z","timestamp":1347342550000},"page":"4046-4054","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Global Effect of Interleukin-10 on the Transcriptional Profile Induced by Neisseria meningitidis in Human Monocytes"],"prefix":"10.1128","volume":"80","author":[{"given":"Unni","family":"Gopinathan","sequence":"first","affiliation":[{"name":"Blood Cell Research Unit, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reidun","family":"\u00d8vsteb\u00f8","sequence":"additional","affiliation":[{"name":"Blood Cell Research Unit, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole Kristoffer","family":"Olstad","sequence":"additional","affiliation":[{"name":"Blood Cell Research Unit, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berit","family":"Brusletto","sequence":"additional","affiliation":[{"name":"Blood Cell Research Unit, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans Christian","family":"Dalsbotten Aass","sequence":"additional","affiliation":[{"name":"Blood Cell Research Unit, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kierulf","sequence":"additional","affiliation":[{"name":"Blood Cell Research Unit, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petter","family":"Brandtzaeg","sequence":"additional","affiliation":[{"name":"Blood Cell Research Unit, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway"},{"name":"Department of Pediatrics, Oslo University Hospital, Oslo, Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Petter","family":"Berg","sequence":"additional","affiliation":[{"name":"Blood Cell Research Unit, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2005.05.003"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.2.4"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"2592","DOI":"10.4049\/jimmunol.153.6.2592","article-title":"IL-1 beta-converting enzyme is present in monocytic cells as an inactive 45-kDa precursor","volume":"153","author":"Ayala JM","year":"1994","unstructured":"AyalaJM. 1994. IL-1 beta-converting enzyme is present in monocytic cells as an inactive 45-kDa precursor. J. Immunol. 153:2592\u20132599.","journal-title":"J. Immunol."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519030090030301"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519010070060401"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.2.195"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.1.51"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.3.650"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0001-2815.2004.00290.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-10-56"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/416190a"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.9.5771"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.1.229"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh083"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01046.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-010-0467-7"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1160\/TH07-06-0424"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1900"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(95)00164-M"},{"key":"e_1_3_3_21_2","doi-asserted-by":"crossref","first-page":"593","DOI":"10.33549\/physiolres.930372","article-title":"Dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by LPS or infected by Borrelia","volume":"52","author":"Jansk\u00fd L","year":"2003","unstructured":"Jansk\u00fdLReymanov\u00e1PKopeck\u00fdJ. 2003. Dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by LPS or infected by Borrelia. Physiol. Res. 52:593\u2013598.","journal-title":"Physiol. Res."},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2008.11.006"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-04-1228"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3630"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.5.2253"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.12.8128"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01043.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.6.2109-2112.1995"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M410114200"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033478"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1080\/003655100750019260"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02664"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00599-3"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-011-1126-6"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0907609"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838415"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500419102"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2006.01.010"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.006353"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-146720"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00635.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.2980-2987.2004"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01625-07"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.4.2380"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-11-21"},{"key":"e_1_3_3_47_2","first-page":"39","article-title":"Bacterial endotoxin: chemical constitution, biological recognition, host response, and immunological detoxification","volume":"216","author":"Rietschel ET","year":"1996","unstructured":"RietschelET. 1996. Bacterial endotoxin: chemical constitution, biological recognition, host response, and immunological detoxification. Curr. Top. Microbiol. Immunol. 216:39\u201381.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181844399"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.09.002"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61016-2"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10759"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03308"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.1.144"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404246200"},{"key":"e_1_3_3_55_2","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1189\/jlb.72.4.800","article-title":"IL-10 expression profiling in human monocytes","volume":"72","author":"Williams L","year":"2002","unstructured":"WilliamsLJaraiGSmithAFinanP. 2002. IL-10 expression profiling in human monocytes. J. Leukoc. Biol. 72:800\u2013809.","journal-title":"J. Leukoc. Biol."}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00386-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00386-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T03:23:57Z","timestamp":1714361037000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00386-12"}},"subtitle":[],"editor":[{"given":"J. N.","family":"Weiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":54,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1128\/IAI.00386-12"],"URL":"https:\/\/doi.org\/10.1128\/iai.00386-12","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]},"assertion":[{"value":"2012-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-08-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}