{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:28:25Z","timestamp":1790166505808,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Infectivity and persistence by\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            , the etiologic agent of Lyme disease, rely stringently on regulatory events. Among these is the downregulation of lipoprotein antigen expression, exemplified by outer surface protein C (OspC), at the advent of specific immunity in the mammalian host.\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            spirochetes that lack the linear plasmid 28-1 (lp28-1) succumb to the host's immune response. We thus explored the notion that these two phenomena were related\u2014that lp28-1\n            <jats:sup>\u2212<\/jats:sup>\n            organisms fail to downregulate\n            <jats:italic>ospC<\/jats:italic>\n            and thus are cleared following the appearance of anti-OspC antibody in the murine host. The lp-28-1\n            <jats:sup>\u2212<\/jats:sup>\n            isolate and a wild-type (wt) isolate bearing the complete set of plasmids were grown in dialysis membrane chambers that were implanted into rat peritoneal cavities. Analysis of mRNA and protein from these cultures showed that OspC expression levels by lp28-1\n            <jats:sup>\u2212<\/jats:sup>\n            organisms are abnormally high in vivo. A time course analysis of\n            <jats:italic>ospC<\/jats:italic>\n            expression in tissues following infection indicates also that temporal diminution of the dominant antigen OspC is impaired in lp28-1\n            <jats:sup>\u2212<\/jats:sup>\n            spirochetes. Finally, passive transfer of monoclonal OspC-specific antibody into SCID mice 8 days postinfection cleared lp28-1\n            <jats:sup>\u2212<\/jats:sup>\n            spirochetes, yet the wt organisms persisted in a majority of animals. These findings indicate that incomplete repression of OspC by lp28-1\n            <jats:sup>\u2212<\/jats:sup>\n            organisms renders them susceptible to immune-mediated clearance. The lp28-1 plasmid must harbor one or more genes involved in OspC downregulation.\n          <\/jats:p>","DOI":"10.1128\/iai.00387-08","type":"journal-article","created":{"date-parts":[[2008,7,15]],"date-time":"2008-07-15T00:34:43Z","timestamp":1216082083000},"page":"3984-3991","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["The Failure of Immune Response Evasion by Linear Plasmid 28-1-Deficient\n            <i>Borrelia burgdorferi<\/i>\n            Is Attributable to Persistent Expression of an Outer Surface Protein"],"prefix":"10.1128","volume":"76","author":[{"given":"Monica E.","family":"Embers","sequence":"first","affiliation":[{"name":"Divisions of Bacteriology and Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Comparative Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara","family":"Ooms","sequence":"additional","affiliation":[{"name":"Veterinary Medicine, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario T.","family":"Philipp","sequence":"additional","affiliation":[{"name":"Divisions of Bacteriology and Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2325"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05895.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.52.2.549-554.1986"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3181-3187.1999"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01698.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5063-5072.2004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3419-3428.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2003.11.014"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4169-4173.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01435-6"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306845101"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3468-3478.2002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.7083-7090.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1714-1718.2000"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.7.3699-3706.2003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4608-4613.2003"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.446-455.2001"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.11.6577-6585.2004"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011870"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020770"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5759-5767.2004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5470-5472.1999"},{"key":"e_1_3_2_25_2","unstructured":"Guide for the care and use of laboratory animals. 1996"},{"key":"e_1_3_2_26_2","first-page":"69","volume":"40","year":"1995","unstructured":"Nocton, J. 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