{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:28:40Z","timestamp":1790616520318,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2023,12,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Streptococcus agalactiae<\/jats:italic>\n            (group B strep, GBS) infections in neonates are often fatal and strongly associated with maternal GBS vaginal colonization. Previously, we highlighted the importance of a formerly uncharacterized protein, BvaP, in GBS vaginal colonization. BvaP is highly conserved across GBS and is made up of repeated domains, with a variable number of repeats between strains. Here, we evaluate the prevalence of BvaP repeated domains and their relevance in phenotypes previously associated with vaginal colonization. Using\n            <jats:italic>in silico<\/jats:italic>\n            analysis, we found that the number of repeats in the BvaP protein does not generally appear to be associated with serotype, isolation site, or host. Using BvaP truncations in GBS strain A909, we determined that a smaller number of repeats was correlated with decreased bacterial chain length, but adherence to vaginal epithelial cells was complemented using BvaP containing one, two, three, or five repeats. Future research will be geared toward understanding the host immune response to BvaP\n            <jats:italic>in vivo<\/jats:italic>\n            and whether vaginal carriage or host response is dependent on the BvaP repeated domains.\n          <\/jats:p>","DOI":"10.1128\/iai.00387-23","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T13:00:59Z","timestamp":1698930059000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["<i>In silico<\/i>\n            and experimental analysis of the repeated domains in BvaP, a protein important for GBS vaginal colonization"],"prefix":"10.1128","volume":"91","author":[{"given":"Lamar S.","family":"Thomas","sequence":"first","affiliation":[{"name":"Department of Biology, Binghamton Biofilm Research Center, Binghamton University, Binghamton, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A.","family":"Faiola","sequence":"additional","affiliation":[{"name":"Department of Biology, Binghamton Biofilm Research Center, Binghamton University, Binghamton, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Canessa","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yetrib","family":"Hathout","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-9570","authenticated-orcid":false,"given":"Laura C.","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Biology, Binghamton Biofilm Research Center, Binghamton University, Binghamton, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.497"},{"key":"e_1_3_5_3_2","first-page":"167","article-title":"Vaginal carriage rate of group B Streptococcus in pregnant women and its transmission to neonates","volume":"22","author":"Chaudhry BY","year":"2010","unstructured":"Chaudhry BY, Akhtar N, Balouch AH. 2010. Vaginal carriage rate of group B Streptococcus in pregnant women and its transmission to neonates. J Ayub Med Coll Abbottabad 22:167\u2013170.","journal-title":"J Ayub Med Coll Abbottabad"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01014-13"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002752200"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002752200"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(00)80234-8"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00480-08"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00933-10"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00762-17"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00421-22"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-584-7:513"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00932-19"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01937"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1003"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnz150"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00202-12"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(96)80561-4"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00094-11"},{"key":"e_1_3_5_20_2","first-page":"175","volume-title":"Proceedings International Conference on Intelligent Systems for Molecular Biology","volume":"6","author":"Sonnhammer EL","year":"1998","unstructured":"Sonnhammer EL, von Heijne G, Krogh A. 1998. A hidden markov model for predicting transmembrane Helices in protein sequences. 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