{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:54:19Z","timestamp":1791327259741,"version":"4.3.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The two-partner secretion (TPS) pathway is widespread among gram-negative bacteria and facilitates the secretion of very large and often virulence-related proteins. TPS systems consist of a secreted TpsA protein and a TpsB protein involved in TpsA transport across the outer membrane. Sequenced\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            genomes contain up to five TpsA- and two TpsB-encoding genes. Here, we investigated the distribution of TPS-related open reading frames in a collection of disease isolates. Three distinct TPS systems were identified among meningococci. System 1 was ubiquitous, while systems 2 and 3 were significantly more prevalent among isolates of hyperinvasive clonal complexes than among isolates of poorly invasive clonal complexes. In laboratory cultures, systems 1 and 2 were expressed. However, several sera from patients recovering from disseminated meningococcal disease recognized the TpsAs of systems 2 and 3, indicating the expression of these systems during infection. Furthermore, we showed that the major secreted TpsAs of systems 1 and 2 depend on their cognate TpsBs for transport across the outer membrane and that the system 1 TpsAs undergo processing. Together, our data indicate that TPS systems may contribute to the virulence of\n            <jats:italic>N. meningitidis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00393-08","type":"journal-article","created":{"date-parts":[[2008,8,5]],"date-time":"2008-08-05T00:40:04Z","timestamp":1217896804000},"page":"4649-4658","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Two-Partner Secretion Systems of\n            <i>Neisseria meningitidis<\/i>\n            Associated with Invasive Clonal Complexes"],"prefix":"10.1128","volume":"76","author":[{"given":"Peter","family":"van Ulsen","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology, Utrecht University, 3584 CH Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Rutten","sequence":"additional","affiliation":[{"name":"Department of Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moniek","family":"Feller","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center, 1100 DD Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Tommassen","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Utrecht University, 3584 CH Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arie","family":"van der Ende","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center, 1100 DD Amsterdam, The Netherlands"},{"name":"The Netherlands Reference Laboratory for Bacterial Meningitis (AMC\/RIVM), 1100 DD Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00079-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0030230"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.6194-6197.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00052.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400291101"},{"key":"e_1_3_2_7_2","first-page":"749","year":"2004","unstructured":"Filloux, A., S. Bleves, P. van Ulsen, and J. Tommassen. 2004. Protein secretion mechanisms in Pseudomonas, p. 749-792. In J. Ramos (ed.), Pseudomonas, genomics, life style and molecular architecture. Kluwer Academic\/Plenum Publishers, New York, NY.","journal-title":"Pseudomonas, genomics, life style and molecular architecture."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.3.775-783.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.03.008"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02278.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-86"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2006.07.013"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.2082-2095.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/35006655"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2007.10.004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00851-07"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28099-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5459.1809"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.8.3156-3161.1992"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.03.007"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00013.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2001.tb00534.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03773.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(82)90015-4"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90365-I"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.1874-1884.2004"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00393-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00393-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:24:08Z","timestamp":1646465048000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00393-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1128\/IAI.00393-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.00393-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10]]},"assertion":[{"value":"2008-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-07-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}