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Both the 97-amino-acid-long KETc7 peptide and its carboxyl-terminal, 18-amino-acid-long sequence (GK-1) are found in\n            <jats:named-content content-type=\"genus-species\">Taenia crassiceps<\/jats:named-content>\n            . Both peptides have proven protective capacity against cysticercosis and are part of the highly conserved, cestode-native, 264-amino-acid long protein KE7. KE7 belongs to a ubiquitously distributed family of proteins associated with membrane processes and may participate in several vital cell pathways. The aim of this study was to identify the\n            <jats:named-content content-type=\"genus-species\">T. solium<\/jats:named-content>\n            KE7 (TsKE7) full-length protein and to determine its immunogenic properties. Recombinant TsKE7 (rTsKE7) was expressed in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            Rosetta2 cells and used to obtain mouse polyclonal antibodies. Anti-rTsKE7 antibodies detected the expected native protein among the 350 spots developed from\n            <jats:named-content content-type=\"genus-species\">T. solium<\/jats:named-content>\n            cyst vesicular fluid in a mass spectrometry-coupled immune proteomic analysis. These antibodies were then used to screen a phage-displayed 7-random-peptide library to map B-cell epitopes. The recognized phages displayed 9 peptides, with the consensus motif Y(F\/Y)PS sequence, which includes YYYPS (named GK-1M, for being a GK-1 mimotope), exactly matching a part of GK-1. GK-1M was recognized by 58% of serum samples from cysticercotic pigs with 100% specificity but induced weak protection against murine cysticercosis.\n            <jats:italic>In silico<\/jats:italic>\n            analysis revealed a universal T-cell epitope(s) in native TsKE7 potentially capable of stimulating cytotoxic T lymphocytes and helper T lymphocytes under different major histocompatibility complex class I and class II mouse haplotypes. Altogether, these results provide a rationale for the efficacy of the KETc7, rTsKE7, and GK-1 peptides as vaccines.\n          <\/jats:p>","DOI":"10.1128\/iai.00395-17","type":"journal-article","created":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T00:40:22Z","timestamp":1505781622000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Experimental and Theoretical Approaches To Investigate the Immunogenicity of Taenia solium-Derived KE7 Antigen"],"prefix":"10.1128","volume":"85","author":[{"given":"Ra\u00fal J.","family":"Bobes","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Navarrete-Perea","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"},{"name":"Instituto Nacional de Medicina Gen\u00f3mica, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adri\u00e1n","family":"Ochoa-Leyva","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edctor Hugo","family":"Anaya","sequence":"additional","affiliation":[{"name":"Escuela Nacional de Estudios Superiores, Unidad Morelia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Morelia, Michoac\u00e1n, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marisela","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacquelynne","family":"Cervantes-Torres","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karel","family":"Estrada","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filiberto","family":"S\u00e1nchez-Lopez","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"Sober\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca, Morelos, M\u00e9xico"},{"name":"Instituto Nacional de Medicina Gen\u00f3mica, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Rosas","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e1ris Maroni","family":"Nunes","sequence":"additional","affiliation":[{"name":"UNESP, Universidade Estadual Paulista, Department of Animal Health and Production, Ara\u00e7atuba, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mart\u00edn","family":"Garc\u00eda-Varela","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rogerio Rafael","family":"Sotelo-Mundo","sequence":"additional","affiliation":[{"name":"Laboratorio de Estructura Biomolecular, Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo, A.C. 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