{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:02:50Z","timestamp":1791165770130,"version":"4.1.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Neisseria meningitidis<\/jats:named-content>\n                    is a commensal of humans that can colonize the nasopharyngeal epithelium for weeks to months and occasionally invades to cause life-threatening septicemia and meningitis. Comparatively little is known about meningococcal gene expression during colonization beyond those first few hours. In this study, the transcriptome of adherent serogroup B\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>\n                    strain MC58 was determined at intervals during prolonged cocultivation with confluent monolayers of the human respiratory epithelial cell line 16HBE14. At different time points up to 21 days, 7 to 14% of the meningococcal genome was found to be differentially regulated. The transcriptome of adherent meningococci obtained after 4 h of coculture was markedly different from that obtained after prolonged cocultivation (24 h, 96 h, and 21 days). Genes persistently upregulated during prolonged cocultivation included three genes (\n                    <jats:italic>hfq<\/jats:italic>\n                    ,\n                    <jats:italic>misR<\/jats:italic>\n                    \/\n                    <jats:italic>phoP<\/jats:italic>\n                    , and\n                    <jats:italic>lrp<\/jats:italic>\n                    ) encoding global regulatory proteins. Many genes encoding known adhesins involved in epithelial adherence were upregulated, including those of a novel locus (spanning\n                    <jats:italic>NMB0342<\/jats:italic>\n                    to\n                    <jats:italic>NMB0348<\/jats:italic>\n                    [\n                    <jats:italic>NMB0342-NMB0348<\/jats:italic>\n                    ]) encoding epithelial cell-adhesive function. Sixteen genes (including\n                    <jats:italic>porA<\/jats:italic>\n                    ,\n                    <jats:italic>porB<\/jats:italic>\n                    ,\n                    <jats:italic>rmpM<\/jats:italic>\n                    , and\n                    <jats:italic>fbpA<\/jats:italic>\n                    ) encoding proteins previously identified by their immunoreactivity to sera from individuals colonized long term with serogroup B meningococci were also upregulated during prolonged cocultivation, indicating that our system models growth conditions\n                    <jats:italic>in vivo<\/jats:italic>\n                    during the commensal state. Surface-expressed proteins downregulated in the nasopharynx (and thus less subject to selection pressure) but upregulated in the bloodstream (and thus vulnerable to antibody-mediated bactericidal activity) should be interesting candidate vaccine antigens, and in this study, three new proteins fulfilling these criteria have been identified: NMB0497, NMB0866, and NMB1882.\n                  <\/jats:p>","DOI":"10.1128\/iai.00397-13","type":"journal-article","created":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T00:13:56Z","timestamp":1377562436000},"page":"4149-4159","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Transcriptional Profiling of Neisseria meningitidis Interacting with Human Epithelial Cells in a Long-Term\n                    <i>In Vitro<\/i>\n                    Colonization Model"],"prefix":"10.1128","volume":"81","author":[{"given":"Ariann","family":"Hey","sequence":"first","affiliation":[{"name":"Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Shi","family":"Li","sequence":"additional","affiliation":[{"name":"Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Hudson","sequence":"additional","affiliation":[{"name":"Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom"},{"name":"Public Health England, Porton Down, Salisbury, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Langford","sequence":"additional","affiliation":[{"name":"Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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