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Prevention of disease could be achieved through improved sanitation and clean water provision supported by vaccination.<jats:italic>V. cholerae<\/jats:italic>serogroup O1 is the major cause of cholera; O1 serotypes Inaba and Ogawa have similar disease burdens, while O139 is the only non-O1 serogroup to cause epidemics. We showed previously that immunization of adult female mice with purified<jats:italic>V. cholerae<\/jats:italic>outer membrane vesicles (OMVs) elicits an antibody response that protect neonates from oral<jats:italic>V. cholerae<\/jats:italic>challenge and that suckling from an immunized dam accounts for the majority of protection from<jats:italic>V. cholerae<\/jats:italic>colonization. Here we report that lipopolysaccharide (LPS) is the major OMV protective antigen. Mucosal immunization with OMVs from Inaba or Ogawa provides significant cross-serotype protection from<jats:italic>V. cholerae<\/jats:italic>colonization, although serotype-specific antigens are dominant. OMVs from O1 or O139 do not provide cross-serogroup protection, but by immunization with a mixture of O1 and O139 OMVs, cross-serogroup protection was achieved. Neonatal protection is not associated with significant bacterial death but may involve inhibition of motility, as antibodies from OMV-immunized mice inhibit<jats:italic>V. cholerae<\/jats:italic>motility<jats:italic>in vitro<\/jats:italic>, with trends that parallel<jats:italic>in vivo<\/jats:italic>protection. Motility assays also reveal that a higher antibody titer is required to immobilize O139 compared to O1, a phenotype that is O139 capsule dependent.<\/jats:p>","DOI":"10.1128\/iai.00398-10","type":"journal-article","created":{"date-parts":[[2010,8,3]],"date-time":"2010-08-03T02:16:02Z","timestamp":1280801762000},"page":"4402-4420","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Mucosal Immunization with<i>Vibrio cholerae<\/i>Outer Membrane Vesicles Provides Maternal Protection Mediated by Antilipopolysaccharide Antibodies That Inhibit Bacterial Motility"],"prefix":"10.1128","volume":"78","author":[{"given":"Anne L.","family":"Bishop","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schild","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bharathi","family":"Patimalla","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Camilli","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.12.036"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.11.7692"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/169.1.230"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/169.3.709"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90481-U"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)66550-6"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.3733-3739.1999"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.09.049"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2009.09.013"},{"key":"e_1_3_2_11_2","first-page":"383","volume":"9","year":"2002","unstructured":"Attridge, S. 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