{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:28:57Z","timestamp":1793118537815,"version":"4.5.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,7]]},"abstract":"ABSTRACT<\/jats:title>Brucella abortus<\/jats:named-content>is an intracellular pathogen that uses a crafty strategy to invade and proliferate within host cells, but the distinct signaling pathways associated with phagocytic mechanisms ofB. abortus<\/jats:named-content>remain unclear. The present study was performed to test the hypothesis that Toll-like receptor 4 (TLR4)-linked signaling interacting with Janus kinase 2 (JAK2) plays an essential role inB. abortus<\/jats:named-content>phagocytosis by macrophages. The effects of TLR4-JAK2 signaling onB. abortus<\/jats:named-content>phagocytosis in murine macrophage RAW 264.7 cells were observed through an infection assay and confocal microscopy. We determined that the uptake ofB. abortus<\/jats:named-content>was negatively affected by the dysfunction of TLR4 and JAK2. F-actin polymerization detected by flow cytometry and F-actin assay was amplified forB. abortus<\/jats:named-content>entry, whereas that event was attenuated by the disruption of TLR4 and JAK2. Importantly, JAK2 phosphorylation and actin skeleton reorganization were suppressed immediately afterB. abortus<\/jats:named-content>infection in bone marrow-derived macrophages (BMDMs) from TLR4\u2212\/\u2212<\/jats:sup>mice, showing the cooperation of JAK2 with TLR4. Furthermore, small GTPase Cdc42 participated in the intermediate pathway of TLR4-JAK2 signaling onB. abortus<\/jats:named-content>phagocytosis. Consequently, TLR4-associated JAK2 activation in the early cellular signaling events plays a pivotal role inB. abortus<\/jats:named-content>-induced phagocytic processes in macrophages, implying the pathogenic significance of JAK2-mediated entry. Here, we elucidate that this specific phagocytic mechanism ofB. abortus<\/jats:named-content>might provide achievable strategies for inhibitingB. abortus<\/jats:named-content>invasion.<\/jats:p>","DOI":"10.1128\/iai.00403-13","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T04:03:46Z","timestamp":1367294626000},"page":"2448-2458","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Toll-Like Receptor 4-Linked Janus Kinase 2 Signaling Contributes to Internalization of Brucella abortus by Macrophages"],"prefix":"10.1128","volume":"81","author":[{"given":"Jin Ju","family":"Lee","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Hyeok","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae Geun","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu Jang","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wongi","family":"Min","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man Hee","family":"Rhee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Youl","family":"Cho","sequence":"additional","affiliation":[{"name":"School of Life Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahisa","family":"Watarai","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suk","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea"},{"name":"Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Zoonoses and communicable diseases common to man and animals","author":"Acha P","year":"1980","unstructured":"AchaPSzylresB. 1980. 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