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The bacterium causing this disease,<jats:italic>Burkholderia pseudomallei<\/jats:italic>, is also considered a bioterrorism agent, and as yet there is no licensed vaccine for preventing<jats:italic>B. pseudomallei<\/jats:italic>infection. In this study, we evaluated selected proteins (LolC, PotF, and OppA) of the ATP-binding cassette systems of<jats:italic>B. pseudomallei<\/jats:italic>as candidate vaccine antigens. Nonmembrane regions of the<jats:italic>B. pseudomallei<\/jats:italic>proteins were expressed and purified from<jats:italic>Escherichia coli<\/jats:italic>and then evaluated as vaccine candidates in an established mouse model of<jats:italic>B. pseudomallei<\/jats:italic>infection. When delivered with the monophosphoryl lipid A-trehalose dicorynomycolate adjuvant, the proteins stimulated antigen-specific humoral and cellular immune responses. Immunization with LolC or PotF protein domains afforded significant protection against a subsequent challenge with<jats:italic>B. pseudomallei<\/jats:italic>. The most promising vaccine candidate, LolC, provided a greater level of protection when it was administered with immune-stimulating complexes complexed with CpG oligodeoxynucleotide 10103. Immunization with LolC also protected against a subsequent challenge with a heterologous strain of<jats:italic>B. pseudomallei<\/jats:italic>, demonstrating the potential utility of this protein as a vaccine antigen for melioidosis.<\/jats:p>","DOI":"10.1128\/iai.00404-07","type":"journal-article","created":{"date-parts":[[2007,5,22]],"date-time":"2007-05-22T16:39:04Z","timestamp":1179851944000},"page":"4173-4180","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Identification of a LolC Homologue in<i>Burkholderia pseudomallei<\/i>, a Novel Protective Antigen for Melioidosis"],"prefix":"10.1128","volume":"75","author":[{"given":"David N.","family":"Harland","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United 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