{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T13:11:09Z","timestamp":1772889069650,"version":"3.50.1"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Extracellular nucleotides are important triggers of innate immunity, acting on a wide variety of cells via signaling through purinergic receptors. Mucosal mast cells contribute to expulsion of a number of gastrointestinal nematode parasites, and mouse mast cell protease 1 has been shown to have a critical role in clearance of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Trichinella spiralis<\/jats:named-content>\n            from the intestinal tract. We show here that adenosine, ADP, ATP, UDP, and UTP all stimulate calcium mobilization in bone marrow-derived mast cells with a mucosal phenotype. Secreted proteins from\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. spiralis<\/jats:named-content>\n            infective larvae inhibit nucleotide-induced mast cell activation, and that induced by ADP and UDP is specifically blocked by parasite secretory 5\u2032-nucleotidase. Release of mouse mast cell protease 1 is stimulated by ADP and ATP. Both parasite secreted products and the 5\u2032-nucleotidase inhibit ADP-induced release of mast cell protease, whereas that stimulated by ATP is partially inhibited by secreted products alone. This indicates that the 5\u2032-nucleotidase contributes to but is not solely responsible for inhibition of nucleotide-mediated effects on mast cell function. Secretion of nucleotide-metabolizing enzymes by parasitic nematodes most likely evolved as a strategy for suppression of innate immune responses and is discussed in this context.\n          <\/jats:p>","DOI":"10.1128\/iai.00411-12","type":"journal-article","created":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T02:32:10Z","timestamp":1344911530000},"page":"3761-3767","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Trichinella spiralis Secreted Enzymes Regulate Nucleotide-Induced Mast Cell Activation and Release of Mouse Mast Cell Protease 1"],"prefix":"10.1128","volume":"80","author":[{"given":"Holly C.","family":"Afferson","sequence":"first","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Eleftheriou","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murray E.","family":"Selkirk","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kleoniki","family":"Gounaris","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00145-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704673"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01571.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.02.014"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-009-9173-z"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601341"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1979.sp013002"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00528.2003"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000061572.10929.2D"},{"key":"e_1_3_3_11_2","first-page":"527","article-title":"International Union of Pharmacology. XXV. Nomenclature and classification of adenosine receptors","volume":"53","author":"Fredholm BB","year":"2001","unstructured":"FredholmBBIJzermanAPJacobsonKAKlotzKNLindenJ. 2001. International Union of Pharmacology. XXV. Nomenclature and classification of adenosine receptors. Pharmacol. Rev. 53:527\u2013552.","journal-title":"Pharmacol. Rev."},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(00)00191-6"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2009.10.024"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.111351"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2330309"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.4917-4924.2002"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.10.005"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2004.04.008"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3658-3662.2001"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1997.0123"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2730"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.2.1013"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119833"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0141-9838.2004.00715.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.12.1849"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.05.005"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.04.004"},{"key":"e_1_3_3_28_2","first-page":"1","article-title":"UTP as an extracellular signaling molecule","volume":"16","author":"Lazarowski ER","year":"2001","unstructured":"LazarowskiERBoucherRC. 2001. UTP as an extracellular signaling molecule. News Physiol. Sci. 16:1\u20135.","journal-title":"News Physiol. Sci."},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1159\/000154944"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1231488100"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1654"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020044"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.141221498"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(96)05696-6"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.10.3473.410k01_3473_3486"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(71)90070-1"},{"key":"e_1_3_3_37_2","first-page":"434","article-title":"Heterogeneity of murine bone marrow-derived mast cells: analysis of their proteinase content","volume":"72","author":"Newlands GF","year":"1991","unstructured":"NewlandsGF. 1991. Heterogeneity of murine bone marrow-derived mast cells: analysis of their proteinase content. Immunology 72:434\u2013439.","journal-title":"Immunology"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.40.1.563"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.4968-4971.2000"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2008.04.028"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)85277-8"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.291666"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1159\/000235327"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.6.1871"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)02094-9"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(03)00193-7"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90115-5"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8253"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2005.08.014"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002100000310"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.2741\/A194"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2002.01233.x"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-009-0647-9"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00411-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00411-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:05:08Z","timestamp":1646481908000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00411-12"}},"subtitle":[],"editor":[{"suffix":"Jr.","given":"J. F.","family":"Urban","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":52,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1128\/IAI.00411-12"],"URL":"https:\/\/doi.org\/10.1128\/iai.00411-12","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]},"assertion":[{"value":"2012-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-07-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}