{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:47:21Z","timestamp":1791352041854,"version":"4.3.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Neisseria meningitidis<\/jats:named-content>\n            is a worldwide cause of meningitis and septicemia leading at least to 50,000 deaths every year. Nevertheless,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>\n            is also a commensal bacterium that asymptomatically colonizes the epithelial cells of the nasopharynx of 10 to 30% of healthy individuals. Occasionally,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>\n            crosses the nasopharyngeal barrier and enters the bloodstream. During bacteremia,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>\n            may adhere to endothelial cells of brain vessels and invade meninges. To identify the genes required for meningococcal host colonization, we screened a signature-tagged transposon mutagenesis library using an innovative\n            <jats:italic>in vitro<\/jats:italic>\n            colonization model in order to identify mutants displaying decreased capacity to colonize human epithelial cells. Approximately 1,600 defined insertion mutants of invasive serogroup C strain NEM8013 were screened. Candidate mutants were tested individually for quantification of bacterial biomass with confocal microscope and COMSTAT software. Five mutants were demonstrated to exhibit significantly decreased colonization ability. The identified genes, including\n            <jats:italic>narP<\/jats:italic>\n            and\n            <jats:italic>estD<\/jats:italic>\n            , appeared to be involved in adaptation to hypoxic conditions and stress resistance. Interestingly, the genes\n            <jats:italic>fadD1<\/jats:italic>\n            ,\n            <jats:italic>nnrS<\/jats:italic>\n            , and\n            <jats:italic>NMV_2034<\/jats:italic>\n            (encoding a putative thioredoxin), prior to this study, had not been shown to be involved in colonization. Therefore, we provide here insights into the meningococcal functions necessary for the bacterium to adapt to growth on host cells.\n          <\/jats:p>","DOI":"10.1128\/iai.00421-13","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T04:30:32Z","timestamp":1372739432000},"page":"3375-3381","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Identification of Genes Involved in Neisseria meningitidis Colonization"],"prefix":"10.1128","volume":"81","author":[{"given":"Anne","family":"Jamet","sequence":"first","affiliation":[{"name":"INSERM U1002, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, Paris, France"},{"name":"Assistance Publique\u2013H\u00f4pitaux de Paris, H\u00f4pital Necker Enfants Malades, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Euphrasie","sequence":"additional","affiliation":[{"name":"INSERM U1002, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Martin","sequence":"additional","affiliation":[{"name":"INSERM, UMR1043, Toulouse, France"},{"name":"INRA, USC 1360, Toulouse, France"},{"name":"CNRS, UMR5282, Toulouse, France"},{"name":"Universit\u00e9 de Toulouse, UPS, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Nassif","sequence":"additional","affiliation":[{"name":"INSERM U1002, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, Paris, France"},{"name":"Assistance Publique\u2013H\u00f4pitaux de 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