{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T01:23:20Z","timestamp":1781227400800,"version":"3.54.1"},"reference-count":70,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,2,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Acinetobacter baumannii<\/jats:italic>\n              is an opportunistic human pathogen that acquires nutrient metals from the vertebrate host amid infection. During zinc (Zn) scarcity,\n              <jats:italic>A. baumannii<\/jats:italic>\n              upregulates the expression of the predicted Zn metallochaperone,\n              <jats:italic>zigA<\/jats:italic>\n              . Loss of\n              <jats:italic>zigA<\/jats:italic>\n              compromises fitness during Zn deficiency, highlighting its role in this condition. To assess the contribution of ZigA to Zn-deficient\n              <jats:italic>A. baumannii<\/jats:italic>\n              , a multiparallel transposon sequencing and genetic interaction mapping approach was used. Transposon insertions in\n              <jats:italic>A1S_3027<\/jats:italic>\n              , encoding a predicted soluble lytic transglycosylase that tailors the bacterial cell wall, were enriched in the Zn-starved \u0394\n              <jats:italic>zigA<\/jats:italic>\n              transposon library. Based on previous studies as well as structural and sequence homology, we designated A1S_3027 as\n              <jats:underline>s<\/jats:underline>\n              oluble\n              <jats:underline>l<\/jats:underline>\n              ytic\n              <jats:underline>t<\/jats:underline>\n              ransglycosylase\n              <jats:underline>B<\/jats:underline>\n              (SltB). Further analyses revealed that inactivating\n              <jats:italic>sltB<\/jats:italic>\n              rescued \u0394\n              <jats:italic>zigA<\/jats:italic>\n              fitness defects during Zn starvation. An\n              <jats:italic>A. baumannii<\/jats:italic>\n              \u0394\n              <jats:italic>zigA<\/jats:italic>\n              \u0394\n              <jats:italic>sltB<\/jats:italic>\n              mutant demonstrated altered cell envelope structures and increased cellular permeability, highlighting the roles of ZigA and SltB in maintaining cell envelope integrity. Furthermore, these mutants exhibited heightened resistance to \u03b2-lactam antibiotics and other cell wall-targeting agents. Alterations in cell envelope integrity in the \u0394\n              <jats:italic>zigA<\/jats:italic>\n              \u0394\n              <jats:italic>sltB<\/jats:italic>\n              mutant improved fitness in a murine pneumonia infection model, emphasizing the contribution of ZigA and SltB to\n              <jats:italic>A. baumannii<\/jats:italic>\n              pathogenesis. This study elucidates how functional interactions between ZigA and SltB modulate cell envelope integrity and pathogenesis of\n              <jats:italic>A. baumannii<\/jats:italic>\n              during Zn depletion. These findings reveal an interplay between metal homeostasis and cell envelope integrity, offering insights into how\n              <jats:italic>A. baumannii<\/jats:italic>\n              ZigA contributes to these critical cellular processes.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00422-24","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T14:00:44Z","timestamp":1737640844000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["The interplay between\n            <i>Acinetobacter baumannii<\/i>\n            ZigA and SltB promotes zinc homeostasis and cell envelope integrity"],"prefix":"10.1128","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3629-8323","authenticated-orcid":false,"given":"Jeanette M.","family":"Critchlow","sequence":"first","affiliation":[{"name":"Microbe-Host Interactions Training Program, Vanderbilt University School of Medicine","place":["Nashville, USA"]},{"name":"Department of Pathology Microbiology and Immunology, Vanderbilt University Medical Center","place":["Nashville, USA"]},{"name":"Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center","place":["Nashville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan P.","family":"Barraza","sequence":"additional","affiliation":[{"name":"Department of Pathology Microbiology and Immunology, Vanderbilt University Medical Center","place":["Nashville, USA"]},{"name":"Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center","place":["Nashville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Munneke","sequence":"additional","affiliation":[{"name":"Microbe-Host Interactions Training Program, Vanderbilt University School of Medicine","place":["Nashville, USA"]},{"name":"Department of Pathology Microbiology and Immunology, Vanderbilt University Medical Center","place":["Nashville, USA"]},{"name":"Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center","place":["Nashville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Krystofiak","sequence":"additional","affiliation":[{"name":"Cell Imaging Shared Resource, Vanderbilt University","place":["Nashville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin R.","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Pathology Microbiology and Immunology, Vanderbilt University Medical Center","place":["Nashville, USA"]},{"name":"Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center","place":["Nashville, USA"]},{"name":"Department of Microbiology, University of Chicago","place":["Chicago, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5094-8105","authenticated-orcid":true,"given":"Eric P.","family":"Skaar","sequence":"additional","affiliation":[{"name":"Department of Pathology Microbiology and Immunology, Vanderbilt University Medical Center","place":["Nashville, USA"]},{"name":"Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center","place":["Nashville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.96.1.39-42.1968"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.03.014"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1196774"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01601"},{"key":"e_1_3_4_6_2","volume-title":"WHO updates list of drug-resistant bacteria most threatening to human health","author":"World Health Organization","year":"2024","unstructured":"World Health Organization. 2024. 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